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Julia M. Harshman, Wayne M. Jurick II, Kim S. Lewers, Shiow Y. Wang and Christopher S. Walsh

). The inoculated raspberries were placed at 25 °C and, once mycelial growth was visualized, the fungus was cultured from the infected fruit onto PDA plates as well as 50-mL potato dextrose broth (PDB) liquid cultures that were used to extract genomic DNA

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S. Laywisadkul, C.F. Scagel, L.H. Fuchigami and R.G. Linderman

decreases the amount of N mobilized from leaves to stems and roots in the autumn; thus, it can reduce N reserves required for growth the following spring ( Bi et al., 2003 ; Guak et al., 2001 ). The combination of foliar sprays with urea and CuEDTA can be

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Joseph N. Wolukau, Xiao-Hui Zhou, Ying Li, Yong-Bin Zhang and Jin-Feng Chen

potato dextrose agar (e.g., Gusmini et al., 2003 ; Kwon et al., 1997 ) in petri plates (150 cm × 30 cm) using mycelial plug inoculation. Petri plates were incubated at 25 °C in the dark for 7 d followed by ultraviolet irradiation (40 w, 12 h·d −1 ) for

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Cláudia S. da Costa Ribeiro and Paul W. Bosland

. Mycelial plugs (0.5 cm in diameter) were taken from the water agar plates and transferred to V8 agar plates that were placed in an incubator at 28 °C for a period of 4 to 8 d. Once the V8 agar was covered with mycelium, the V8 agar was sliced into 12 pieces

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Ryan S. Donahoo, William W. Turechek, Judy A. Thies and Chandrasekar S. Kousik

zoospores were observed, suspensions were passed through two layers of paper tissue (Kimwipe; Kimberly-Clark, Roswell, GA) to eliminate sporangial/mycelial fragments. The flooding/dislodging process was repeated once, at which point the sporangial suspension

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Luisa Santamaria, Emmalea G. Ernest, Nancy F. Gregory and Thomas A. Evans

race F–resistant ‘Maffei 15’ and B2C resulted in plants with three different disease reactions. Some plants were completely resistant, some were very susceptible (i.e., exhibited obvious mycelial growth and died), whereas others were stunted, had some

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Marcelo A.G. Carnelossi, Edinaldo O.A. Sena, Adrian D. Berry and Steven A. Sargent

number of fruit (10 fruit per clamshell) with any incidence of visible mycelial growth, or mechanical damage, was recorded and expressed in percent. Destructive analyses Pulp firmness. Whole fruit firmness was measured at 0, 7, 14, and 21 d for each fruit

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Maria I. Villarroel-Zeballos, Chunda Feng, Angela Iglesias, Lindsey J. du Toit and James C. Correll

d. Erlenmeyer flasks (1 L) containing sterile vermiculite amended with minimal medium nutrient solution ( Korolev and Katan, 1997 ) were each inoculated with 10 mycelial plugs taken from the actively growing edge of a colony of VD1. Non

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Jareerat Chunthawodtiporn, Theresa Hill, Kevin Stoffel and Allen Van Deynze

Bosland and Lindsey (1991) . Each isolate was inoculated individually and freshly cultured on V8-agar for 4 to 5 d. Agar plugs with mycelial growth were excised and placed in a sterile petri dish with sterile distilled water. Cultures were incubated at 25

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Rachel E. Rudolph, Lisa W. DeVetter, Inga A. Zasada and Cedar Hesse

quality were unaffected by the growth of adjacent cover crops compared with that of raspberry growing adjacent to cultivated, bare soil (Till). There are conflicting yield results from previous studies conducted in Canada. British Columbia is often