Abstract
Continuous, low intensity, far red (FR) irradiation prevented germination, and continuous, low intensity, red (R) irradiation decreased the rate of germination of seed of dw-2 dwarf watermelon [Citrullus lanatus (Thunb.) Matsumara and Nakai]. Intermittent, 15 minute light treatments with R or FR at 6 hour intervals affected germination similarly to continuous irradiations. Seeds germinated best in darkness and would germinate in darkness following prolonged incubation in FR light. A short exposure to R light following a prolonged FR treatment enhanced subsequent germination in darkness (D), and the effect of R light was reversed by a short exposure to FR light, indicating phytochrome control of germination. Responses to single, short exposures to FR light after different periods of incubation, and to different intervals of D and prolonged FR light, indicated that germination of dwarf watermelon seeds is regulated by phytochrome between about 6 to 24 hours of incubation at 29 to 30°C.
Abstract
Seeds of 29 terrestrial orchid species representing 15 genera were surface sterilized by immersion in 0.5% sodium hypochlorite containing a wetting agent, washed, sown on a completely defined, semisolid embryo culture medium containing macro- and microelements, sucrose, amino acids, and vitamins, and incubated in the dark at 25°C. Six months after sowing, 16 species from 9 genera germinated and continued development while 13 species from 10 genera failed to germinate. Species of Cypripedium, Goodyera, Platanthera and Spiranthes differed in response in that one or more of each germinated and one or more did not. Seedling development was similar for most germinating species and progressed to the formation of a shoot or shoot initial in all but one. Apparently the mycorrhizal association thought to be required for terrestrial orchid seed germination and early seedling development can be replaced with aseptic culture on a completely defined medium for many terrestrial orchids.
The autointoxication of chrysanthemum was studied using water extract of Dendranthema morifolium's rhizospheric soil. Results of bioassays showed that the water extract inhibited chrysanthemum seed germination and the activities of some important root enzymes. The seedling nitrate reductase activity was decreased linearly with increasing concentration of the extract. The activity of root dehydrogenase was inhibited only at the highest concentration tested [3.2 g·mL-1, dry weight (DW)], but was stimulated at a lower concentration tested (1.6 g·mL-1, DW). Malondialdehyde content increased at higher than 1.6 g·mL-1, DW concentrations of the extract. The autointoxication phenomenon might be related to the difficulties in continuous plantings of chrysanthemum at the same location.
Temperatures above 30 °C may delay or inhibit germination of most of commercial lettuce cultivars. Ethylene enhances lettuce seed germination at high temperatures. Enzyme-mediated degradation of endosperm cell walls appears to be a crucial factor for lettuce germination at high temperature. The galactomannan polysaccharides in lettuce endosperm cell wall are mobilized by endomannanase. The role of endo-mannanase during germination of lettuce seeds at high temperature (35 °C) and the possible role of etlene in enzyme regulation were investigated. Seeds of thermotolerant (`Everglades'-EVE) and thermosensitive (`Dark Green Boston'-DGB) lettuce genotypes were incubated at 20 and 35 °C in water, 10 mM of 1-aminocyclopropane-1-carboxylic acid (ACC), or 20 mM of silver thiosulphate (STS). Also, seeds were primed in an aerated solution of polyethylene glycol (PEG), or PEG+ACC, or PEG+STS. Untreated seeds germinated 100% at 20 °C. At 35 °C, EVE germinated 100%, whereas DGB germinated only 33%. Seed priming or adding ACC during imbibition increased germination of DGB to 100% at 35 °C. Adding STS during imbibition led to a decrease in germination at 35%C in EVE and completely inhibited germination of DGB. Priming with STS led to reduced germination at 35%C of both genotypes. EVE produced more ethylene than DGB during germination at high temperature. Providing ACC either during priming or during germination led to an increase in endo-mannanase activity, whereas STS inhibited mannanase activity. Higher endo-mannana activity was observed in EVE than DGB seeds. The results suggest that ethylene might overcome the inhibitory effect of high temperature in thermosensitive lettuce seeds via weakening of endosperm due to increased endo-mannanase activity.
Poor peach seed germination can be a problem for commercial tree fruit nurseries. Even standard rootstocks such as Lovell and Nemaguard do not always have high germination rates. New seed-propagated rootstocks under development, such as Guardian peach rootstock, often are selected for their field traits, with nursery characteristics being of secondary importance. Guardian rootstock is derived from bulked open-pollinated seed from a number of F1 seedling selections. Germination of Guardian bulked seed has been poor. Four pre-stratification cold treatments were given to four 100-seed lots each of Lovell, Nemaguard, and 10 Guardian selections prior to planting each year (1994 to 1998) in a Cecil sandy loam at Musser Fruit Research Center near Clemson, S.C. Treatments included taking dry, refrigerated seed that were harvested in August and soaking 100-seed seedlots in 1500 mL perlite and 400 mL distilled water for 0 (no soaking), 2, 4, 6, and 8 weeks at 6 °C before sowing (typically early November). All treatments consisted of 25 seeds per replicate (4 reps/year) per rootstock or selection and were sowed the same day. The experiment was analyzed as a blocked split plot design with duration of stratification the whole-plot and seedlot the sub-plot. Number of emerged seedlings were counted weekly starting in January of each year. There were significant differences between stratification treatments, seedlots and years. The 6-week pre-stratification had the highest germination over 5 years and like the 8-week treatment advanced the average germination date by 20 to 30 days. Nemaguard (65%), Lovell (64%), and Guardian 3-17-7 (60%) had the best germination percent across all treatments and years, with SL2891 (42%) slightly less. All other selections averaged less than 25%. Year-to-year variation was large, indicating strong environmental influences on seed germination despite the pre-stratification treatments.
The influence of increasing levels (0.0%, 0.05%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1.2%, 1.6%, and 2.0%) of NaCl on the germination of Kentucky bluegrass (Poa pratensis), annual ryegrass (Lolium multiflorum), perennial ryegrass (Lolium perenne), creeping bentgrass (Agrostis palustris), tall fescue (Festuca arundinacea), and crested wheatgrass (Agropyron cristatum) was investigated. Kentucky bluegrass, creeping bentgrass, and crested wheatgrass had a 50% reduction in germination at 0.2%, 0.6%, and 0.6% NaCl, respectively, compared to the control and completely lost germination at 0.6%, 1.2%, and 1.6% NaCl, respectively. Seed germination in both annual ryegrass and perennial ryegrass was only 50% of the control at 1.2% NaCl and completely inhibited at 2.0% NaCl. Tall fescue, red fescue, and creeping red fescue showed a 50% reduction in germination at NaCl concentrations of 1.2%, 1.2%, and 0.8%, respectively, while showing a complete inhibition of germination at 2.0%, 2.0%, and 1.6% NaCl, respectively.
Iris versicolor (blue-flag iris) is a native aquatic plant that grows from Maine to Virginia. It is an important species of wetland regeneration and restoration. Unfortunately, seed germination seldom occurs in the wild. To address this problem, seeds of Iris versicolor were soaked with gibberellin acid (0, 500, 1000, and 1500 ppm) for 24 h after 120 days of cold treatment at 4 °C and then were randomly assigned to three germination temperatures (constant 21 °C; 24 °C/18 °C; 27C/15 °C) and placed in darkness. Germination rates for the three temperature treatments were 54.4% (21 °C), 96.5% (24 °C/18 °C), and 96.0% (27C/15 °C). Oscillating temperature treatments had significantly greater germination rate than constant temperature. Gibberellin acid had significant influence on germination rate; only the constant 21 °C was not favorable for germination. The germination rate was higher at 1000 than at 500 ppm or 1500 ppm or more. Germination occurred within 10 days under germination temperature treatments. All seedlings in petri dishes were successfully transplanted into growing flats.
The seeds of difficult-to-germinate native species of American licorice (Glycyrrhiza lepidota Pursh), angelica (Angelica atropurpurea L.), wild blueberry (Vaccinium angustifolium Ait.), wild mint (Mentha arvensis L.), and purple coneflower (Echinacea angustifolia D.C.), were soaked in 0, 2.5, 5.3, or 7.6 m KOH solutions for 0, 1, 5, 10, 15, or 20 minutes. After these treatments, germination was determined at 10 °C with a 14-hour photoperiod in petri dishes and seedling emergence was conducted at 14/10 °C with a 14-hour photoperiod in a sandy loam soil. In general, KOH treatment for only 1 min enhanced germination and seedling emergence. The optimal KOH concentration and soaking time in improving seed germination and seedling emergence varied with species, being 5.3 m and 10 min for purple coneflower, 5.3 m and 5 minutes for wild blueberry, 7.6 m and 5 minutes for angelica, 7.6 m and 1 minute for wild mint, and 5.3-7.6 m and 10 minutes for American licorice, respectively. Prolonged soaking, particularly in high concentrations, reduced germination and emergence.
The effect of seed germination rate, or of seedling emergence rate, was studied in relation to subsequent plant growth of `Cortina' lettuce (Lactuca sativa L.). Seedling growth response to selection by time of germination was assessed by imbibing seeds at 5 °C to increase the time range for germination. Germinated seeds were removed daily and transferred to “slants” (germination paper held at 20° from vertical) at 15 °C. Five days after each transfer, root and hypocotyl lengths were measured. As days required for germination increased, root lengths decreased and hypocotyl lengths increased, resulting in no change in total seedling length. The relation between rate of seedling emergence from raw or pelleted seeds of the same lot and shoot fresh weight was examined using commercially practiced hydroponic techniques. Shoot fresh weight at 10 and 21 days after planting was related inversely and linearly to the day of emergence for both seed treatments. In the same study, the coefficient of variation of shoot fresh weight was positively related to time of seedling emergence only at 10 days. Germinated seeds were selected after 1 and 2 days of imbibition; subsequent seedling emergence rate and shoot fresh weight at 25 days were recorded. First-day germinated seeds had faster and more synchronous emergence, and produced heavier and more uniform shoots. Discarding slow-to-germinate seeds should enhance seedling emergence and growth.
Seeds of Lupinus havardii Wats. (Big Bend bluebonnet), a potential cut flower crop, were subjected to a variety of scarification and temperature treatments. Without scarification, only 10-20% of the seeds germinated within one week. Germination percentages increased sigmoidally as scarification time in concentrated sulfuric acid increased. Nearly 100% germination was obtained within one week after seeds were placed in sulfuric acid for 120 min. Nicking the seed coat with a razor blade also resulted in near 100% germination. Soaking the seed in water for 24 h failed to enhance germination. Soaking the seed in ethanol, methanol, or acetone for 2 h likewise failed to enhance germination. Total germination of scarified seed was >90% between 21 and 33C within 28 h. The most rapid germination occurred within a range of 24-29C. Above or below this range germination was delayed. At 35C, seedling, mortality was observed and total germination was reduced to <50%. Our data indicate that seed of this species requires scarification for optimum germination but the seed can germinate over a relatively wide temperature range.