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Huseyin Karlidag, Ertan Yildirim, Metin Turan, Mucahit Pehluvan and Figen Donmez

leakage (membrane permeability) For measurement of electrolyte leakage, 10 leaf discs (10 mm in diameter) from the young fully expanded leaves from two plants per replicate were placed in 50-mL glass vials and rinsed with distilled water to remove the

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Ao Liu, Jibiao Fan, Margaret Mukami Gitau, Liang Chen and Jinmin Fu

; Moradi and Ismail, 2007 ). It has been documented that the ROS can lead to a significant cell membrane damage manifesting in the form of increased malonaldehyde (MDA) content, increased electrolyte leakage (EL), and pronounced activity of antioxidases

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Smiljana Goreta, Daniel I. Leskovar and John L. Jifon

( Turner, 1981 ). The RWC expressed as percent was calculated as Electrolyte leakage. Leaves of about the same age as those sampled for ψ lf and RWC measurements were used for the electrolyte leakage (EL) determination by a modified procedure

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Sukhvinder Pal Singh and Zora Singh

-reactive substances, lipoxygenase activity, and electrolyte leakage. Thiobarbituric acid-reactive substance (TBARS) concentration was determined as previously described ( Singh et al., 2012 ) and was expressed as nanomoles malondialdehyde equivalents per gram of fresh

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Mohamad Hossein Sheikh Mohamadi, Nematollah Etemadi, Ali Nikbakht and Mohammad Pessarakli

osmotic adjustments ( Ervin and Koski, 2001 ; McCann and Huang, 2007 ). Xu and Huang (2011) have shown the effect of TE on increasing leaf RWC under drought stresses. Electrolyte leakage. Traffic stress significantly increased the EL of tall fescue and

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Yiming Liu, Hongmei Du, Kai Wang, Bingru Huang and Zhaolong Wang

, distilled water was supplied to compensate the transpiration loss by weighing the pots daily. Measurement of turf quality, relative water content, and electrolyte leakage. Turf quality, leaf RWC, and EL were measured to compare overall turf performance under

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Diego Barranco, Natividad Ruiz and María Gómez-del Campo

This study aims to determine the relationship between laboratory frost-resistance data for the leaves of eight olive cultivars and observed field resistance in the same genotypes undergoing natural frost damage. The lethal freezing temperature (LT50) for each cultivar was established by measuring the electrical conductivity (EC) of the medium into which solutes from damaged leaf tissue were leaked. The value obtained was then correlated with percentage frost shoot for the same eight cultivars damaged by natural frosts in a field test. A negative correlation was observed between the percentage frost shoot and leaf LT50 for all the cultivars under study. The most frost-hardy cultivars (`Cornicabra', `Arbequina', and `Picual') were those presenting the lowest percentage frost shoot and lowest LT50. Conversely, the most frost-susceptible cultivar (`Empeltre') displayed 100% frost shoot, together with one of the highest LT50 values (–9.5 °C). According to these results, lethal freezing temperature (LT50) calculated from leaf ion leakage at a range of freezing temperatures, seem to be a valid parameter for evaluating frost tolerance in olive cultivars.

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Martha Edith López-López, José Ángel López-Valenzuela, Francisco Delgado-Vargas, Gabriela López-Angulo, Armando Carrillo-López, Lidia Elena Ayón-Reyna and Misael Odín Vega-García

, 2 = 26% to 50% tissue injury, 3 = 51% to 75% tissue injury, and 4 = ≥76% of tissue injury). The CII for each fruit was calculated by the following expression: CII = (ILL + ILP + ILU + ILD)/4. Electrolyte leakage. The method described by Malacrida et

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Liang Cheng, Ning Zhang and Bingru Huang

plants ( Fig. 5B ). Fig. 5. Electrolyte leakage of Burkholderia gladioli RU1- and Burkholderia phytofirmans PsJN-inoculated and noninoculated perennial ryegrass under ( A ) nonstressed control condition and ( B ) salinity condition. Vertical bars

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Lixin Xu, Mili Zhang, Xunzhong Zhang and Lie-Bao Han

, 14, and 21 d ( Fig. 1A ). At 21 d, cold treatment increased MDA by 80% when compared with the control. Fig. 1. Leaf electrolyte leakage (EL; mean ± se , n = 10) and malondialdehyde (MDA content) responses to cold acclimation treatment in ‘Chinese