Instruments, Wilmington, DE) 5 min at 10,000 g n at 4 °C. Supernatant (3 mL) was removed to a test tube, to which 1 mL derivatizing solution, 1,2-benzenediamine dihydrochloride, was added (final Ph, 2.20–2.45), vortexed for 5 s, filtered through a 45-μm
20 m m potassium phosphate (pH 2.5 by phosphoric acid) at a flow rate of 1 mL/min and were detected at 210 nm. L-Proline (Sigma) dissolved in the 20 mm potassium phosphate solution was used to calibrate the standard curve. The amount of proline in
of 5.8 ( Lien and Rognes, 1997 ); a pH increase from 3.5 to 9.2 causes a reduction in glycine uptake in Lolium perenne ( Thornton, 2001 ), and glycine uptake by Pisum sativum shows little change between pH 2 to 9 ( Dureja et al., 1984 ). Glycine
globe, red, medium-firm, green shoulder, avg. 175 g. Determinate. Resistance: Verticillium wilt race 1, Fusarium wilt races 1 and 2, early blight (stems), late blight (Ph2 and Ph3 heterozygous.) Adaptation: temperate. 2011. Five Star Grape —Breeder and
; attractive; very good flavor; soluble solids usually 11-12 °Brix; pH 2.8 titratable acidity 6.8 g·L –1 ; ripens mid-early on floricanes, 3 d before Ouachita in Arkansas; very good postharvest potential and recommended for commercial shipping. Plant: erect