ploidy level of plants was detected using a flow cytometer (Partec, Münster, Germany). Young leaves of plants obtained from anther culture were put into petri dishes containing 400 µL extraction buffer (Partec 05–5002 CyStain ® ultraviolet Precise P
Chromosome doubling has been widely explored as a bridge to help gene introgression between different ploidy levels both between and within species for crop improvement ( Hayward et al., 1993 ; Meru, 2012 ; Wu et al., 2011 ). Both fruit and seed
features. Development of in vitro regeneration systems provides an ideal foundation for further improvements by ploidy manipulations, mutation treatments, and transgenic applications. Previous in vitro regeneration studies of Hypericum have focused on
-cedar ( Chiba, 1951 ) was used to select 237 seedlings for transplantation. After 1 month of growth [180 d after treatment (DAT)], the ploidy level of the seedlings was determined using flow cytometry. Identification of polyploidy. Flow cytometry, as
that were 20% to 70% higher ( Cameron and Hartley, 1990 ). In addition to the octoploid species, several lower-ploidy Fragaria species (2×, 4×, and 6×) have desirable characteristics such as extreme vigor ( Harbut et al., 2009 ), unique flavors
these chemicals for chromosome doubling varies depending on the plant species, concentration levels, and treatment duration. Restored fertility, increased flower and leaf sizes, and shortened internodes often accompany ploidy manipulation ( Pryor and
cytometric analysis was used to determine ploidy level as was done for C. sativa by Bagheri and Mansouri (2015) and Parsons et al. (2019) . For flow cytometric analysis, 50 mg of young leaf tissue was harvested per plant. A modified version of the
/or acidic fruit that were greater than 50 g at harvest. Trees that appeared unhealthy or had insufficient fruit for analyses were excluded. Ploidy data were obtained from a 20,000 single-nucleotide polymorphism (SNP) array as part of an ongoing collaborative
as well as across ploidy levels have been documented, including M . × giganteus (2 n = 3 x = 57), a naturally occurring triploid hybrid between diploid M. sinensis and tetraploid M. sacchariflorus ( Glowacka and Jezowski, 2009 ). As a result
evaluate the plant performance, growth, flowering, fruit production, seed viability, and ploidy level of the wild-type and eight additional new heavenly bamboo cultivars planted in southern Florida (Fort Pierce, USDA plant hardiness zone 9b) and northern