Search Results

You are looking at 101 - 110 of 436 items for :

Clear All

colchicine treatment. Chromosome counting. The ploidy level was determined by chromosome counting in metaphasic cells ( Rego et al., 2011 ). Chromosome detection was performed as described by Chen et al. (1979) . Two months after colchicine treatment, stem

Free access

) supplemented with gibberellic acid, which promotes shoot elongation. When shoots were ≈1 to 2 cm long, individual elongated shoots were moved to RMA rooting medium in Magenta boxes for further hardening and growth. Emergent shoots were analyzed for their ploidy

Free access

Chromosome doubling has been widely explored as a bridge to help gene introgression between different ploidy levels both between and within species for crop improvement ( Hayward et al., 1993 ; Meru, 2012 ; Wu et al., 2011 ). Both fruit and seed

Free access

ploidy level of plants was detected using a flow cytometer (Partec, Münster, Germany). Young leaves of plants obtained from anther culture were put into petri dishes containing 400 µL extraction buffer (Partec 05–5002 CyStain ® ultraviolet Precise P

Free access

features. Development of in vitro regeneration systems provides an ideal foundation for further improvements by ploidy manipulations, mutation treatments, and transgenic applications. Previous in vitro regeneration studies of Hypericum have focused on

Free access

-cedar ( Chiba, 1951 ) was used to select 237 seedlings for transplantation. After 1 month of growth [180 d after treatment (DAT)], the ploidy level of the seedlings was determined using flow cytometry. Identification of polyploidy. Flow cytometry, as

Free access

that were 20% to 70% higher ( Cameron and Hartley, 1990 ). In addition to the octoploid species, several lower-ploidy Fragaria species (2×, 4×, and 6×) have desirable characteristics such as extreme vigor ( Harbut et al., 2009 ), unique flavors

Free access

these chemicals for chromosome doubling varies depending on the plant species, concentration levels, and treatment duration. Restored fertility, increased flower and leaf sizes, and shortened internodes often accompany ploidy manipulation ( Pryor and

Free access

cytometric analysis was used to determine ploidy level as was done for C. sativa by Bagheri and Mansouri (2015) and Parsons et al. (2019) . For flow cytometric analysis, 50 mg of young leaf tissue was harvested per plant. A modified version of the

Open Access

/or acidic fruit that were greater than 50 g at harvest. Trees that appeared unhealthy or had insufficient fruit for analyses were excluded. Ploidy data were obtained from a 20,000 single-nucleotide polymorphism (SNP) array as part of an ongoing collaborative

Open Access