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El-Sayed Mohamed El-Mahrouk, Eman Abdel-Hakim Eisa, Mahmoud Abdelnaby Hegazi, Mohamed El-Sayed Abdel-Gayed, Yaser Hassan Dewir, Mohammed Elsayed El-Mahrouk and Yougasphree Naidoo

by adding 100 μL of crude enzyme extract. Changes in absorbance at 470 nm were recorded at 30-s intervals for 3 min. Enzyme activity was expressed as an increase in the absorbance min −1 ·g −1 fresh weight. Electrolyte leakage. Measurements were

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Longxing Hu, Tao Hu, Xunzhong Zhang, Huancheng Pang and Jinmin Fu

GB did not affect on EL and MDA content under non-salinity conditions. Fig. 4. Effects of glycine betaine (GB) on leaf electrolyte leakage (EL) ( A ) and lipid peroxidation (MDA) ( B ) of perennial ryegrass under salt stress or non-stress conditions

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Vijaya Shukla, Yingmei Ma and Emily Merewitz

infrared thermometer was used to measure canopy temperature (Model 2956; Spectrum Technologies). Leaf electrolyte leakage (EL) was measured by taking ≈200 mg of leaf samples from each plant. The tissue was briefly rinsed in deionized water and then put into

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Sanalkumar Krishnan, Kevin Laskowski, Vijaya Shukla and Emily B. Merewitz

the leaf electrolyte leakage (EL). Approximately 10 leaves were taken from each plant and washed with deionized water for three times and immersed in 25 to 30 mL of deionized water and placed in a shaker for 24 h. The conductivity of the water

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Yong In Kuk and Ji San Shin

expressed as changes in conductivity relative to an initial measurement. The electrolyte leakage levels recorded just before chilling were used as initial measurements. All treatments were replicated three times, and conductivity values were expressed on a

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Ustun Sahin, Melek Ekinci, Fatih Mehmet Kiziloglu, Ertan Yildirim, Metin Turan, Recep Kotan and Selda Ors

(EC2) was measured. Electrolyte leakage was calculated as a percentage of EC1/EC2. Leaf relative water content. LRWC was measured according to Gonzalez and Gonzalez-Vilar (2001) . The young fully expanded leaves were first removed from stem and

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Diheng Zhong, Hongmei Du, Zhaolong Wang and Bingru Huang

completely desiccated and RWC reached the same level in both genotypes (29% in ‘Tifway’ and 28% in ‘C299’). Fig. 1. Changes in leaf relative water content ( A ) and electrolyte leakage ( B ) during drought stress in drought-resistant hybrid bermudagrass

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Huseyin Karlidag, Ertan Yildirim, Metin Turan, Mucahit Pehluvan and Figen Donmez

leakage (membrane permeability) For measurement of electrolyte leakage, 10 leaf discs (10 mm in diameter) from the young fully expanded leaves from two plants per replicate were placed in 50-mL glass vials and rinsed with distilled water to remove the

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Ao Liu, Jibiao Fan, Margaret Mukami Gitau, Liang Chen and Jinmin Fu

; Moradi and Ismail, 2007 ). It has been documented that the ROS can lead to a significant cell membrane damage manifesting in the form of increased malonaldehyde (MDA) content, increased electrolyte leakage (EL), and pronounced activity of antioxidases

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Diego Barranco, Natividad Ruiz and María Gómez-del Campo

This study aims to determine the relationship between laboratory frost-resistance data for the leaves of eight olive cultivars and observed field resistance in the same genotypes undergoing natural frost damage. The lethal freezing temperature (LT50) for each cultivar was established by measuring the electrical conductivity (EC) of the medium into which solutes from damaged leaf tissue were leaked. The value obtained was then correlated with percentage frost shoot for the same eight cultivars damaged by natural frosts in a field test. A negative correlation was observed between the percentage frost shoot and leaf LT50 for all the cultivars under study. The most frost-hardy cultivars (`Cornicabra', `Arbequina', and `Picual') were those presenting the lowest percentage frost shoot and lowest LT50. Conversely, the most frost-susceptible cultivar (`Empeltre') displayed 100% frost shoot, together with one of the highest LT50 values (–9.5 °C). According to these results, lethal freezing temperature (LT50) calculated from leaf ion leakage at a range of freezing temperatures, seem to be a valid parameter for evaluating frost tolerance in olive cultivars.