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Konstantinos G. Batziakas, Shehbaz Singh, Kanwal Ayub, Qing Kang, Jeffrey K. Brecht, Cary L. Rivard and Eleni D. Pliakoni

life was evaluated by measuring overall quality, water loss, leaf surface color, leaf tenderness, electrolyte leakage, chlorophyll fluorescence, and chlorophyll content. Overall quality. Spinach overall quality was measured similar to Medina et al

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Jiwon Jeong, James Lee and Donald J. Huber

each fruit half. Electrolyte leakage. Mesocarp cylinders (5 cylinders per fruit) 8 mm in diameter were removed from the equatorial mesocarp tissue using a cork borer. The cylinders were trimmed to produce disks of 8-mm thickness using the

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Shinsuke Agehara and Daniel I. Leskovar

. They suggested that reductions in stomatal conductance ( g S ) by ABA enabled the maintenance of leaf water potential and prevented increases in electrolyte leakage and leaf abscission. On the other hand, Latimer (1992) reported that root

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Hongmei Du, Zhaolong Wang and Bingru Huang

). Measurements. Turf quality was rated based on canopy color, density, and uniformity on the scale of 0 to 9 with 9 being the best (green, dense canopy) and 0 being the worst (dead turf) ( Beard, 1973 ). Turfgrass visual quality, electrolyte leakage

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Chae Shin Lim, Seong Mo Kang, Jeoung Lai Cho and Kenneth C. Gross

lycopersicum L. ( Autio and Bramlage, 1986 )], mandarin [ Citrus reticulata Blanco ( Lafuente et al., 1997 )], and pepper ( Lim et al., 2007 ; Lin et al., 1993 ). Ethylene production, respiration, and electrolyte leakage (EL) usually precede or occur

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Zhimin Yang, Jingjin Yu, Emily Merewitz and Bingru Huang

cellular membrane stability was evaluated by measuring electrolyte leakage of leaves ( Blum and Ebercon, 1981 ). Fresh leaves (0.1–0.2 g) were collected, rinsed, and immersed in 20 mL deionized water and placed on a conical shaker at 23 °C for 24 h. The

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Qin Shi, Yunlong Yin, Zhiquan Wang, Wencai Fan and Jianfeng Hua

sulfosalicylic acid and heated at 100 °C for 10 min. Concentration of proline was then determined in this extract by acid-ninhydrin reagent method on a DW basis ( Bates et al., 1973 ). Relative electrolyte leakage. A total of 0.2 g fresh leaf was immersed in a

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Lijian Liang, Yanming Deng, Xiaobo Sun, Xinping Jia and Jiale Su

/ F m represents the maximum F v / F m of photosystem II (PS II), and was calculated according to Krüger et al. (1997) . Membrane permeability and lipid peroxidation determination. Membrane permeability, expressed by relative electrolyte leakage

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Hongmei Du, Zhaolong Wang, Wenjuan Yu and Bingru Huang

photochemical efficiency analyzer (OS 1FL; Opti-Sciences, Hudson, NH) after dark adaptation for 30 min. Cell membrane stability of leaves was estimated using the measurement of electrolyte leakage from leaf cells ( Blum and Ebercon, 1981 ). EL of leaves was

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Cong Li, Lie-Bao Han and Xunzhong Zhang

)]. The RWC was determined as follows: RWC = (FW – DW)/(TW – DW) × 100. Leaf malondialdehyde and electrolyte leakage measurement. Leaf tissues (50 mg) of WT and transgenic tobacco plants were homogenized in 1 mL of 80% ethanol and centrifuged at 16,000 g