Mycorrhizal colonization can alter stomatal behavior of host leaves during drought. This may be related to an altered production or reception of a chemical signal of soil drying. We tested whether intact root systems were required to observe a mycorrhizal effect on leaf transpiration (E), or whether some residual mycorrhizal influence on leaves could affect E of foliage detached from root systems. Transpiration assays were performed in the presence of several possible candidates for a chemical signal of soil drying. In detached leaves of Vigna unguiculata (cowpea), colonization interacted significantly with ABA and pH in regulating transpiration. Colonization affected E of detached Rosa hybrida (rose) leaves but had no effect on E of detached leaves of Pelargonium hortorum (geranium). In each species tested, increasing the ABA concentration decreased E. In cowpea, calcium appeared to alter stomatal sensitivity to ABA, as well as regulate stomatal activity directly. The pH of the feeding solution affected E in rose, but did not change E independently in cowpea or geranium. Adding phosphorus to the feeding solution did not alter E or the apparent sensitivity of stomata to ABA in any of the test species. Colonization of roots by mycorrhizal fungi can result in residual effects in detached leaves, that can alter the stomatal reception of chemical signals in both rose and cowpea.
Craig D. Green, Ann Stodola and Robert M. Augé
Mark P. Kaczperski, Allan M. Armitage and Pamela M. Lewis
Pelargonium×hortorum L.H. Bailey `Scarlet Elite' seedlings were grown in plugs from seed to transplant size. About 14 days before attaining transplant size, seedlings were exposed to various fertility or temperature regimes (preconditioning treatments), then stored for 1 to 3 weeks at 5C. Seedlings receiving 150 mg N/liter before storage flowered sooner and required less crop time (days to flower – days in storage) than those receiving 0, 75, or 300 mg. Temperature preconditioning at 10 or 15C delayed flowering compared to preconditioning at 20C. Final plant height and dry weight were not adversely affected by varying N levels or temperature during preconditioning. Preconditioning seedlings with 300 mg N/liter resulted in seedling mortality rates up to 16% after 7 days' storage. Low temperature or fertility were not effective preconditioning treatments. Best results were attained by preconditioning seedlings with 150 mg N/liter.
Mark P. Kaczperski, Royal D. Heins and William H. Carlson
Methods of cold storage for rooted cuttings of three cultivars of Pelargonium ×hortorum Bailey were examined. Cuttings were stored from 0 to 10°C for 7 to 56 days. Treatments included packing the cuttings in ice, storing them under irradiance levels of 0 or 50 μmol·m–2·s–1, applying fungicides, varying cutting developmental stages, and varying the day temperatures. Cuttings packed in ice showed signs of chilling injury within 7 days and died. Applications of etridiazole and thiophanate-methyl or metalaxyl and thiophanate-methyl drenches or fosetyl-Al spray did not improve storage performance of the cuttings. Roots of cuttings held 7 additional days in the propagation area before storage grew faster after storage than those of cuttings with less time in the propagation area, but flowering time was not affected. Maintaining night temperatures at 5°C while allowing day temperatures to rise to 10°C delayed flowering by 6 days compared to maintaining a constant 5°C. Rooted cuttings held at 5°C under 50 μmol·m–2·s–1 irradiance for 9 hours each day could be stored up to 56 days with only a 2-day delay in flowering compared to unstored cuttings. Chemicals used were 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazole (etridiazole); thiophanate-methyl (dimethyl[1,2-phenylene)bis(iminocarbonothioyl)]bis[carbamate]) (thiophanate-methyl); N-(2,6-dimethylphenyl)-N-methoxyacetyl) alanine methyl ester (metalaxyl); aluminum tris (O-ethyl phosphonate) (fosetyl-Al).
K.G. Childs, T.A. Nell, J.E. Barrett and D.G. Clark
Experiments were conducted to evaluate the development of stored unrooted Pelargonium × hortorum `Designer Bright Scarlet' cuttings. Treatments included storage temperature and duration and pre-storage fungicide application. Cuttings were harvested from stock plants treated with water or fungicide (Iprodione), and were stored at 60°F and 75°F for 2, 4, and 6 days. Leaf yellowing data (visual quality rating, chlorophyll fluorescence, and total chlorophyll content) were measured at the start of propagation and 7 days later. At both dates, cuttings stored but not treated with fungicide displayed more leaf yellowing after storage at 75°F for 4 and 6 days or at 60°F for 6 days compared to fungicide-treated cuttings and non-stored controls. Cutting quality was not affected by 2 days of storage, regardless of storage temperature or fungicide treatment. Fungicide-treated cuttings had less leaf yellowing after storage for 6 days at 60°F or 75°F compared to untreated cuttings, but they had more leaf yellowing than no storage controls after 7 days of propagation. Root number and root length of each cutting was measured at 14 days after start of propagation. Cuttings treated with fungicide displayed better adventitious root formation after all 4- and 6-day storage treatments compared to cuttings stored but not treated with fungicide.
Mark P. Kaczperski and Royal D. Heins
Plug-grown Pelargonium × hortorum `Pinto Red' seedlings were grown under natural daylight (average of 4.7 mol/day) or with supplemental irradiance from high-pressure sodium lamps. Seedlings were grown under 8-, 16-, or 24-h photoperiods with supplemental irradiances of 2.5, 3.75, or 5.0 mol/day at each photoperiod. Supplemental irradiance was provided for 7, 14, 21, and 28 days beginning 7, 14, 21, 28, and 35 days after sowing. Seedlings were transplanted 63 days after sowing to 8-cm containers (121 plants/m2) and grown to flower. Leaf number at time of transplant was not affected by photoperiod, but increased as daily irradiance and weeks of supplemental irradiance increased. Seedlings were more responsive to supplemental irradiance applications beginning 28 and 35 days than at 7 to 21 days after sowing. Ninety-two percent of seedlings receiving 28 days of 5.0 mol/day supplemental irradiance under a 24-h photoperiod starting 35 days after sowing had initiated flower buds at time of transplant; 75% of those receiving 3.75 mol/day were initiated. Plants receiving less than 3 weeks of supplemental irradiance or with an irradiance period beginning less than 28 days after sowing had not initiated flowers at transplant.
L.A. Gladstone and G.W. Moorman
Jaime K. Morvant, John M. Dole and Janet C. Cole
Pelargonium ×hortorum Bailey `Pinto Red' plants were fertilized with equal amounts of N, P, and K derived from: 1) 100% constant liquid fertilization (CLF); 2) 50% CLF plus 50% controlled-release fertilizer (CRF); or 3) 100% CRF per pot and irrigated using hand (HD), microtube (MT), ebb-and-flow (EF), or capillary mat (CM) irrigation systems. The treatment receiving 100% CRF produced greater total dry weights, and released lower concentrations of NO3-N, NH4-N, and PO4-P in the run-off than the 100% CLF treatment. The percentage of N lost as run-off was greatly reduced with the use of CRF. MT irrigation produced the greatest plant growth and HD irrigation produced the least. The EF system was the most water efficient, with only 4.7% of water lost as run-off. Combining the water-efficient EF system with the nutrient-efficient CRF produced the greatest percentage of N retained by plants and medium (90.7) and the lowest percentage of N lost in the run-off (1.7).
L.A. Gladstone and G.W. Moorman
Jeff S. Kuehny, Matt Taylor and Michael R. Evans
) that are composed of pressed wood pulp and a minimum of 74% recycled paper, with at least 37% post-consumer recycled (on dry weight basis) ( Fig. 1 ). Growth of marigold ( Tagetes erecta ), vinca, impatiens, geranium, and tomato ( Solanum lycopersicum
Nicole L. Waterland, Craig A. Campbell, John J. Finer and Michelle L. Jones
; 41.6 cm 3 /cell) and seed geraniums (finished 10-cm pots). All plants except the seed geraniums were transplanted to 11-cm pots containing soilless greenhouse media (Promix BX; Premier Horticulture, Quebec, Canada). Plants were grown under normal