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- Author or Editor: Zhongquan Qiao x
Weigela florida (Bunge) A. DC. is a popular flowering shrub adapted to a wide range of environmental conditions. Efficient methods for micropropagation of this species have not been well developed. The present study established a protocol for in vitro shoot culture of W. florida ‘Tango’ after a systematic evaluation of different culture media, cytokinins, and auxins on axillary shoot induction. Single-node stems were cultured on Driver and Kuniyuki Walnut (DKW) medium for initial production of axillary shoots. The shoots were used as explants and cultured on DKW medium supplemented with 8.88 μm 6-benzylaminopurine (BA) and 0.27 μm naphthaleneacetic acid (NAA), resulting in the production of more than six axillary shoots per explant. The axillary shoots could either be used as explants for additional shoot production or be cultured on ½ DKW medium supplemented with 0.25 μm indole-3-butyric acid (IBA) for rooting. Plantlets were transplanted into a substrate with 99% survival rate in a shaded greenhouse. This established method could be used for rapid propagation of W. florida to speed the introduction of new hybrids or cultivars for commercial production.
Lagerstroemia indica (crape myrtle) is a popular Chinese landscape plant with a long flowering period that contributes to its gorgeous flowers and high ornamental value, which motivate L. indica breeding. We found a wild acarpous individual of L. indica that did not bear seeds after flowering and had a significantly longer flowering period than fructiferous L. indica. This study identified differences in floral organ morphology, and stamen and pistil structure between fructiferous and acarpous L. indica through observation, paraffin sectioning, and scanning electron microscopy (SEM). The flowering time of each acarpous L. indica inflorescence lasts as long as 18 to 25 days. When a single flower withers, it falls from the pedicel without any fruit. The abortion in the floral organ of acarpous L. indica is characterized by sterile and undehisced anthers, pollen abortion, and deformed and irregularly arranged filament cells. Acarpous L. indica features short and loosely arranged papilla cells in the stigma, a flat style and narrow stylar canal, loosely arranged epidermal cells, and no obvious nuclei. No embryo sac cavity is found in acarpous L. indica ovules. In some nucelli, the egg apparatus structure can be observed indistinctly but without cell contour. In others, the egg apparatus structure is completely absent, and only flocculent tissue is observed. This study may provide a theoretical foundation for future studies on the molecular mechanisms of the mutations in acarpous L. indica.