Clonal plants can consist of connected individual ramets that enhance resource sharing through physiological integration. This integration enables the whole clone to tolerate environmental stresses. The objective of this research was to investigate the effects of physical ramet connections on the integration of antioxidant enzymes in clonal common bermudagrass (Cynodon dactylon) growing under heterogeneously distributed water; i.e., nonuniform distribution of water due to 20% polyethylene glycol (PEG 6000) treatment on some ramets and not others. The bottom, middle, upper and three fragments of clonal common bermudagrass were subjected to 20% PEG 6000 with water potential of −1.8 MPa to induce heterogeneous and homogeneous drought stress. The control was not treated with 20% PEG 6000. Within the heterogeneous treatment, water stressed clonal fragments generally had higher leaf and root antioxidant enzyme activities with respect to superoxide dismutase, catalase, peroxidase (except for root peroxidase). There was no difference in antioxidant enzyme activity within the connected clonal ramets for homogeneous treatment; i.e., three connected ramets treated with 20% PEG 6000. Osmotically stressed clonal fragments under heterogeneous environments had a lower level of malonaldehyde (MDA) compared with those in homogeneous regimes. The antioxidant enzyme integration was affected by directionality and water availability contrast. This was indicated by significant decline in MDA levels within the heterogeneous treatments as compared with homogeneous treatment, which suggested reduced lipid peroxidation. These results suggested that ramet connections facilitate integration of antioxidant enzymes within clonal plants growing in heterogeneously available water. Enzymes were integrated from clonal fragments growing in water sufficient environment to those in water stressed regimes. This enhanced reactive oxygen species scavenging capacity of the entire clone hence improved drought tolerance.
Erick Amombo, Longxing Hu, Jibiao Fan, Zhengrong Hu and Jinmin Fu
Zhengrong Hu, Erick Amombo, Margaret Mukami Gitau, Aoyue Bi, Huihui Zhu, Liang Zhang, Liang Chen and Jinmin Fu
Bermudagrass (Cynodon dactylon) is a typical and widely used warm-season turfgrass. Low temperature is one of the key environmental stress limiting its utility. However, little information is available about the differences of cold response between bermudagrass genotypes. Here, we analyzed antioxidant defense system and fatty acid composition in cold-resistant genotype WBD128 and cold-sensitive genotype WBDg17 exposed to chilling stress. Low temperature (4 °C) significantly decreased the relative water content, whereas increased the H2O2 and O2 − contents, more profoundly for WBDg17. Under chilling condition, WBD128 had higher anti O2 − activity than WBDg17. Besides, the contents of total glutathione, reduced glutathione (GSH) and its oxidized form (GSSG) were markedly increased by low temperature in both genotypes, whereas WBD128 had significantly higher values of GSH, total glutathione, and GSH/GSSG ratio than WBDg17. Moreover, chilling stress increased saturated fatty acids (SFAs) percentage (palmitic acid and stearic acid) in WBDg17. After chilling treatment, the proportion of linoleic acid decreased in both genotypes, particularly in WBDg17. As for unsaturated fatty acids (UFAs), the percentage of linolenic acid was increased in WBD128. In addition, chilling treatment decreased the values of double bond index (DBI), UFA/SFA ratio as well as degree of unsaturation in WBDg17. Finally, chilling stress altered the expression patterns of the genes, which encode one kind of late embryogenesis abundant proteins (LEA), superoxide dismutase (Cu/Zn SOD) C-repeat-binding factor/DRE-binding factor (CBF1), and peroxidase (POD-2). Collectively, our results revealed that natural variation of chilling tolerance in bermudagrass genotypes may be largely associated with the alterations of antioxidant defense system and fatty acid composition.