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- Author or Editor: Yvonne M. Page x
Dierama latifolium N.E. Br. was propagated in vitro using corm cultures. Shoots were induced from corm explants when grown on solidified Murashige and Skoog (MS) medium supplemented with 30 g/liter sucrose, 100 mg/liter meso-inositol, and 0 or 0.5 mg/liter NAA. Shoot proliferation was not improved by the addition of BA to the initial culture medium. Multiple shoots were induced by transferring those produced in vitro, to a modified MS medium supplemented with 0.5 or 1.0 mg/liter BA. Rooting of these shoots was induced by subculturing single excised shoots, 5-10 mm in height, either a hormone-free basal medium or a BM supplemented with 0.5 or 1.0 mg/liter NAA. Utilizing this technique, about 90 plants could be produced from a single corm within 12 months. Chemical names used: 1-napthalenacetic acid (NAA); N-(phenylmethyl)-1H-purin-6-amine(BA).
In vitro propagation of geraldton wax (Chamelaucium uncinatum Schauer) was achieved using nodal explants excised from actively growing shoots of adult plants. Murashige and Skoog basal medium (BM) supplemented with 4.4 μΜ BA was optimum for enhanced axillary shoot growth. Repeated subculturing of in vitro-grown axillary shoots resulted in a propagation rate of 5.7 ± 1.1 (±SE) shoots every 6 weeks. Sixtytwo percent of the shoots obtained by this method rooted on half-strength BM supplemented with 58.4 mM sucrose and 5.0 μΜ NAA. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), 1H-indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA).