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Date palm (Phoenix dactylifera L.) is a major tree crop in arid regions of the Middle East and North Africa, having an important impact on the economy of many countries in these regions. Date palms are traditionally propagated through offshoots. The development of propagation methods through tissue culture resulted in massive expansion of date palm plantations. While most trees generated from tissue culture are normal and true-to-type, several typical abnormal phenotypes are detected. The present study applies amplification fragment length polymorphism (AFLP) analysis to characterize the genetic variation of two elite date cultivars, `Barhee' and `Medjool', as well as male clones, propagated from offshoots and through tissue culture. The two cultivars have very distinct AFLP band patterns. Most offshoots, as well as the tissue culture-propagated plants, have very similar band patterns, demonstrating a low level of genetic variation. However, a significant level of genetic variation was detected among `Medjool' plants generated from tissue culture. Several phenotypically abnormal trees were characterized by unique and different AFLP band patterns. The male clones are characterized by a high level of polymorphic bands. Genetic variation was also detected between various tissues of variegated `Medjool' trees propagated from tissue culture. The significance of these results, regarding the mechanism of the phenomenon and its relevance to agricultural practice, is discussed.
Severe damage has been observed in Israeli date palm (Phoenix dactylifera L.) plantations of the Medjool cultivar as a result of drying and dropping of fruit bunches. Both fractures and rot occurred during peduncle (fruit stalk) development at proximal points located deep within the crown. The phenomenon has been previously described as “cross–cut” or “V-cut.” The present study aimed to characterize the phenomenon and identify the main causal factors. Annual surveys have revealed high levels of fruit bunch drop in all ‘Medjool’ cultivation areas in Israel. Arenipses sabella and other insects were only occasionally detected in the damaged fruit stalks. Similar damage levels observed in insecticide-treated and untreated plots suggested that insects were not directly responsible for this phenomenon. Tissue decay was detected in many of the freshly fallen fruit stalks. The pathogenic fungus, Fusarium proliferatum, was detected in most of the necrotic fruit stalks. Fusarium proliferatum isolates from infected fruit stalks generated necrotic lesions in fruit stalk tissue in vitro. However, fungicide treatments on trees in the orchards were ineffective in reducing bunch drop. On the other hand, restraining the growth rate of the fruit stalk, by reducing irrigation levels (to 20%) during the period of fruit stalk development and growth, significantly reduced bunch drop levels in most of the examined plots. These data suggest that bunch drop in ‘Medjool’ date palm is caused mainly by physiological disorders related to fruit stalk development and growth.