Mutation breeding is considered to be economic and efficient in plant improvement, and the use of chemical mutagens such as ethyl methanesulfonate (EMS) can potentially address plant breeding challenges. The aim of this study was to induce morphological mutants in C. indicum var. aromaticum using EMS treatments with different doses, and to analyze the morphological and physiological traits of obtained mutants in expectation of finding favorable mutants. Results revealed significant effects of EMS doses on seed germination. The sample germination rate significantly decreased with increasing of EMS doses. The obtained morphological mutants were two viable types, containing leaf and stem mutants. Overall leaf size was significantly larger as a result of EMS treatments. And the height of mutant plants was significantly higher. Anatomical characteristics exhibited changes in both leaves and stems of the mutant plants. The puncture strength of the bent stem from the mutant plants was low, with weak penetration resistance. The total lignin and cellulose contents of mutant plants stem decreased significantly as a result of the EMS treatments. These results demonstrate the efficiency of EMS to induce mutations in C. indicum var. aromaticum, and this method can be useful in the future to assist breeding of this plant.
Nuananong Purente, Bin Chen, Xiaowei Liu, Yunwei Zhou and Miao He
Yu Liu, Miao He, Fengli Dong, Yingjie Cai, Wenjie Gao, Yunwei Zhou, He Huang and Silan Dai
The NAC transcription factor is a peculiar kind of transcription factor in plants. Transcription factors are involved in the expression of plant genes under different conditions, and they play a crucial role in plant response to various biotic and abiotic stress. We transferred the ClNAC9 gene into Chrysanthemum grandiflora ‘niu9717’ by Agrobacterium tumefaciens–mediated transformation. The results of kanamycin-resistant screening, polymerase chain reaction (PCR) detection, and Northern blot analysis proved that the target gene had been integrated into the genome of the target plants. Wild-type (WT) plants and transgenic plants were treated with different concentrations of NaCl, NaHCO3, and drought stress, and physiological indexes, such as antioxidant system activity (superoxide dismutase, peroxidase, catalase), malondialdehyde accumulation, and leaf relative water content, were measured. We also observed changes in plant morphology. The physiological indexes’ changing range and extreme values suggested that transgenic plants’ resistance to salinity, alkali, and drought stress was significantly higher than WT plants. Transgenic plant growth was less inhibited compared with WT plants, indicating that the ClNAC9 gene increased the resistance of transgenic plants under the stress of salinization, alkalization, and drought.