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Three-river Plain, the key agriculture and animal husbandry developing region of China is located in the northeast part of Heilongjiang province. There were rich resources of wild ornamental plants in Three-river Plain. 134 species of those were investigated belonging to 54 families. 35 were woody plants and 99 herbaceous. The domestication and cultivation of several plants with high decorative value were studied and briefed as follows: (1) Vibrunnum sargenti Koehne propagates by seeds beginning to blossom in 3 years and by rhizome. The plants grown in the plain are subject to insect pests: (2) Sambucus buergeriana Blume propagates by seeds after sand cultivation for 4-6 months and begins to blossom in the next year; (3) Acanthopanax Senticocus (tupr. et (maxim) harmes propagates by seeds, shoot layering and root cutting. The germination of seeds is lower and the shoot layering is best. It grows slowly in the white clay soil, (4) Hemerocallis midendorffii Tranty. et Mey, propagates by seeds and suckers and blossoms in the next year, growing well in the plain; (5) Ostrich fern (Matteuccia struthiopteris (L.) Todaro) propagates by spore. The spore grows to sporophyte in 70-90 days after sowing and the sporophyte grows to sporophyll in 4 years.
An improved three-stage protocol for plant regeneration via somatic embryogenesis of the horticulturally important plant Iris germanica L. was developed using shoot apex segments as explants. At the first stage of the experiment, 60% of callus was obtained from shoot apex segments of I. germanica on Murashige and Skoog’s (MS) medium supplemented with 4.52 μm 2,4-dichloropheoxyacetic acid (2,4-D) and 0.44 μm 6-benzyladenine (6-BA). When nonembryogenic calli were subcultured on MS medium with 11.31 μm 2,4-D and 0.44 μm 6-BA, maximum frequency of embryogenic callus (66.0%) was obtained. At the second stage, the treatment of 9% (w/v) sucrose resulted in the optimum somatic embryo (SE) formation (70.0%). More than 90.0% of SEs germinated with bipolar structure and regenerated into plantlets on plant growth regulator-(PGR)free MS medium during the third stage. Regenerated plantlets were successfully acclimatized in greenhouse environment with little somaclonal variation. Histological study showed that somatic embryogenesis stages were asynchronous and SEs developed from the surface and inner tissue of embryogenic calli.
Stevia rebaudiana Bertoni was hydroponically raised on the matrices of sand or slag and sprinkled periodically with three different nutrient solutions (BD, KO, Knop) respectively. The conventional raising method of Hailin state farm was used as the control. The results showed that the seedlings grown on the matrix of sand and sprinkled with Knop nutrient solution were stronger with well-developed root systems, obvious spindle-shaped root tubers, and less plant diseases, no insect pests, and weeds, which was significantly better than the control method in respect to the root length, root fresh weight, stem height, shoot fresh weight, and number of leaves, and significantly better than other treatments in respect to the root length, root fresh weight and stem height. This raising method is worth extending.