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Guangxin Liu, Yue Lan, Haoyang Xin, Fengrong Hu, Zhuhua Wu, Jisen Shi and Mengli Xi

Lily (Lilium L.) species produce among the most important cut flowers worldwide. China has ≈55 species of Lilium. Although many plants from this genus have been used in hybridization efforts, their cytology has remained unclear. The goal of the current study was to characterize the chromosomes of Lilium rosthornii Diels. Root tips were used to characterize Giemsa C-banding, propidium iodide (PI) banding, and 45S rDNA locations. The karyotype of L. rosthornii belongs to type 3B. C-banding revealed polymorphic banding patterns with the following formula: 2n = 24 = CI = 4C + 14CI+ + 2I+ +2I+ 2. Two of the four 45S rDNA hybridization sites were located at pericentromeric positions on the two short arms of the homologues of chromosome 1, and the other two were located on the long arms of one chromosome 6 homolog and one chromosome 11 homolog. Six of the eight PI bands were detected in the centromeres of the homologues of chromosomes 1, 5, and 8, and the other two PI bands were detected on the long arms of one chromosome 6 and one chromosome 11. Lilium rosthornii showed enriched banding in both Giemsa C-banding and PI painting. Interestingly, not all 45S rDNA was located in homologous chromosomal locations. These results may provide reference data for L. rosthornii for use in further Lilium breeding.

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Guangxin Liu, Xiaoling Zhang, Yue Lan, Haoyang Xin, Fengrong Hu, Zhuhua Wu, Jisen Shi and Mengli Xi

Karyotype comparison and fluorescence in situ hybridization (FISH) were conducted to analyze the wild Lilium species distributed in China. The karyotype results revealed that all species except Lilium lancifolium (2n = 3X = 36) were diploid and had two pairs of metacentric or submetacentric chromosomes. The karyotypes of all species are similar. FISH analysis revealed that there are 5–12 45S rRNA gene loci dispersed on the chromosomes of the 14 diploid species, and 15 45S rRNA gene loci were detected in the triploid species L. lancifolium. Most of the FISH signals were detected on the long arms and the centromeric regions. Three samples of L. brownii [Hubei, China (lat. 31°28′N, long. 110°23′E); Liaoning, China (lat. 40°07′N, long. 124°19′E); and Guangxi, China (lat. 25°06′N, long. 107°27′E)] showed very similar chromosome patterns in both the karyotype and the FISH analyses, further demonstrating that these samples belonged to the same species. L. brownii is widely distributed in China from latitude 25°06′N to 40°07′N, indicating that it is highly adaptable to the environment.

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Chengyan Yue, R. Karina Gallardo, Vicki A. McCracken, James Luby, James R. McFerson, Lan Liu and Amy Iezzoni

Rosaceous crops (e.g., almond, apple, apricot, caneberry, cherry, pear, peach, plum, rose, and strawberry) contribute to human health and well-being and collectively constitute the economic backbone of numerous North American rural communities. We conducted a survey of U.S. and Canadian rosaceous fruit crop breeders to assess priority setting in their programs, sources of information for setting priorities, and challenges in making technical and management decisions. Input from producers and consumers was most important in establishing breeding program targets, although respondents’ direct interaction with consumers was not frequent. Breeding targets and management decisions were mostly associated with the breeder’s type of organization, scope and range of crops, and intended use of the crop (fresh, processed, or both).

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Xiao-Juan Wei, Jinlin Ma, Kun Wang, Xiao-Jing Liang, Jin-Xuan Lan, Yue-Juan Li, Kai-Xiang Li and Haiying Liang

Camellia chrysantha flowers are in great market demand as a result of their high ornamental and medicinal values. To induce early flowering in 4-year-old juvenile C. chrysantha seedlings, three levels of paclobutrazol (PBZ) concentration (100, 200, and 300 ppm) were applied to the roots. PBZ is a triazole-type cytochrome P450 inhibitor that was found successful in inducing flowering in juvenile C. chrysantha grafted plants in a prior report. The current study shows that all three PBZ concentrations were equally effective in induction of floral buds, resulting in an average of 20 floral buds per treated plant. In comparison, none of the untreated plants flowered. Although the induced flowers were smaller than the ones from mature trees, PBZ treatment did not affect C. chrysantha flowers’ medical values, because there was no significant change in the content of pharmacologically active compounds (polysaccharide, polyphenols, flavonoids, and saponins). None of the PBZ treatments had a negative effect on the current year’s growth in height and basal diameter, photosynthesis, and levels of water-soluble sugars and nutrients [phosphorus (P), nitrogen (N), potassium (K), and carbon (C)]. It is concluded that PBZ is an effective flowering inducer for juvenile C. chrysantha plants. It was also found that PBZ-treated plants experienced defoliation, and there existed a strong correlation between severity of defoliation and PBZ concentration. This might be attributed by the stress induced by PBZ, as demonstrated by the increased activities of some of the stress-related enzymes [ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD)], and the level of malondialdehyde (MAD). Considering that severe defoliation can cause stunted or malformed plants and reduce aesthetic value, 100 ppm is the optimal PBZ concentration for flowering induction in C. chrysantha seedlings.