Muskmelon is a warm season cucurbit species that belongs to family Cucurbitaceae. We analyzed 2955 papers of global research on muskmelon (Cucumis melo L.) based on Web of Science from 1924 to 2021. Papers were mainly written in English (2766, 93.604%), from 7883 authors, 83 countries or regions, 1697 organizations, and published in 585 journals and book series. The top five journals were HortScience (334, 11.303%), Journal of the American Society for Horticultural Science (Proceedings of the American Society for Horticultural Science) (117, 3.959%), Scientia Horticulturae (109, 3.689%), Plant Disease (88, 2.978%), and Phytopathology (84, 2.843%), each of which published more than 84 papers. The top five countries or regions were the United States, PR China, Spain, Brazil, and Japan, which each published more than 168 papers. The top six organizations were U.S. Department of Agriculture Agricultural Research Service, Agricultural Research Organization, Texas A&M University, Universitat Politecnica de Valencia, University of California–Davis and Consejo Superior de Investigaciones Cientificas, each of which published more than 64 papers. The top five authors are G.D. Lester, J.D. McCreight, J. Garcia-Mas, K.M. Crosby, and T.J. Ng, who each published more than 26 papers. On the basis of the analysis of a network map of VOSviewer, there were cooperation among authors, organizations, and countries or regions. All keywords of muskmelon research were separated into eight clusters for different research topics. Visualizations offer exploratory information on the current state and indicate possible developments in the future. This work is also useful for students identifying graduate schools and researchers selecting journals.
Bao-Zhong Yuan, Zhi-Long Bie, and Jie Sun
Huan Xiong, He Sun, Feng Zou, Xiaoming Fan, Genhua Niu, and Deyi Yuan
Castanea henryi is an important woody grain tree species native to China. The objective of the current study was to find the suitable plant growth regulators (PGRs) and the optimal concentrations for direct organogenesis by using axillary shoots and cotyledonary nodes. Seeds were collected from the field, sterilized, and germinated in vitro. Axillary shoots and cotyledonary nodes of 3-week-old seedlings were used as explants. To find the suitable PGR for adventitious shoot induction, 0.5 mg·L–1 6-benzylaminopurine (6-BA), 0.1 mg·L–1 indole-3-acetic acid (IAA), 0.1 mg·L–1 2,4-dichlorophenoxyacetic acid (2,4-D), or 0.1 mg·L–1 1-naphthaleneacetic acid (NAA) was supplemented to Murashige and Skoog (MS) medium containing 0.65% agar and 3% sucrose. A high induction percentage of adventitious shoots (85.67%) was obtained from cotyledonary nodes supplemented with 0.1 mg·L–1 2,4-D. The type of explant influenced shoot proliferation rates and quality. Apical explants produced more and longer shoots than nodal segments. For shoot multiplication, 1 mg·L–1 6-BA + 0.05 mg·L–1 indole-3-butyric acid (IBA) supplemented with MS medium produced 12.33 and 6.25 shoots per explant, respectively, from apical and nodal explants. For shoot elongation and strengthening, 2 mg·L–1 6-BA + 0.05 mg·L–1 IBA supplemented with MS medium was the best combination, producing shoots with a mean length of 3.50 cm, a diameter of 0.46 cm, and about eight leaves per shoot. The greatest rooting of 76.70% and 11.33 roots per shoot was achieved when cultured in MS medium supplemented with 3.5% perlite + 1.5 mg·L–1 IBA. For acclimatization of the rooted plantlets in the greenhouse, a survival rate of 80% was achieved. This protocol—from multiplication to acclimation—is helpful to realize mass propagation of high-quality trees of chinquapin for increasing production and nut quality.
Yuanyuan Miao, Qiaosheng Guo, Zaibiao Zhu, Xiaohua Yang, Changlin Wang, Yuan Sun, and Li Liu
The stolon is the main asexual reproductive organ of the medicinal plant Tulipa edulis and has special morphology. In the greenhouse experiment presented herein, the dynamic changes in carbohydrates and related enzymes, proteins, and endogenous hormones of stolons during T. edulis stolon formation were investigated. The results showed that the content of total soluble sugar, sucrose, reducing sugar, fructose, and starch were all significantly enhanced in the middle period when stolon emerged and maintained at relatively high levels until the later period of stolon formation, while protein content decreased during stolon formation. The activities of amylase (AMY), sucrose phosphate synthase (SPS), and sucrose synthase (SS) peaked in the initial period and were negatively correlated with soluble sugars. However, adenosine diphosphoglucose pyrophosphorylase (AGPase) activity increased as stolon formation progressed, and the changes in soluble starch synthase (SSS), granule-bound starch synthase (GBSS) activities presented a single peak, reaching their maximums in the middle period. AGPase, SSS, and GBSS activities were all positively related to starch content. Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) verified the changes in SS and SSS activities via the expression levels of the SS, SSSI, and SSSII genes. The gibberellin (GA) and zeatin riboside (ZR) content attained their maximum in the initial period of stolon formation. Indole-3-acetic acid (IAA) and abscisic acid (ABA) remained at high levels during the initial and middle period and decreased significantly during the later period of stolon formation, inversely to the ratio of ABA:IAA. Analysis of the physiological changes in T. edulis stolon indicated that the accumulation of soluble sugars and starch via various enzymes, a high level of IAA and a low ABA to IAA ratio mainly contributed to stolon development of T. edulis. This paper explored carbohydrate levels and endogenous hormones profiles during stolon formation, which provided the theory basis for further regulating stolon growth of T. edulis.