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The effect of N source on `Kennebec' potato (Solanum tuberosum L.) tuberization was investigated using single-node segments originated from in vitro virus-free plantlets and inoculated on media with two nitrate: ammonia ratios (low, 2:1; high, 5:1). Cell count and size and sugar-use characteristics were measured at intervals of tuber development. Tubers grown on high nitrate-ammonia medium exhibited higher rates of sucrose use and higher dry-matter accumulation than tubers grown on low nitrate-ammonia medium. The median value of tuber fresh weight increased from 0.66 to 1.23 g as a result of increasing nitrate-ammonia. Significant differences in cell size and growth rate were observed between the two N treatments. There was also a high correlation between tuber cell size and dry matter (r = 0.82, P ≤ 0.05). These data demonstrate the importance of the nitrate: ammonium ratio in determining C use, tuber cell size, and tuber weight. Chemical names used: α-naphtylacetic acid (NAA); 6-benzylaminopurine (BA).
Paphiopedilum Clair de Lune ‘Edgard Van Belle’, an excellent Maudiae-type hybrid that has been propagated by artificial division for a long time. We studied its flower bud initiation, development of floral organs, and flowering habits with a view to providing information for flowering control and efficient commercial production. According to our research, the flower bud initiation phase of this cultivar begins in February every year, and 80% of the plants completed sepal primordium differentiation in March, The flower bud differentiation lasts for 6 to 7 months, until flowering in August. Within 1 to 3 months after flower bud differentiation, all tested plants differentiated lateral buds. After 5 to 6 months, the new, aboveground vegetative shoots reached their maximum growth, with an average plant height of 20 cm, five leaves, and a shoot dry weight of more than 3 g. From February to April of the following year, a new cycle of flower development and vegetative growth began. In addition, this cultivar was notably sensitivity to high ambient temperature during the late phase of flower development, with a flower bud drop rate as high as 33.3% under average day/night temperatures of 29.0/26.5 °C.