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  • Author or Editor: Yrina P. Ferreras x
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Shoot proliferation of Acer ginnala Maxim. (Amur maple) from nodal-segments was obtained on Murashige and Skoog salts and vitamins supplemented with 25 nM thidiazuron and 3% sucrose. Higher concentrations of cytokinin resulted in callus formation at the base of explants. Explant orientation had a significant effect on shoot elongation. Explant elongation and proliferation were correlated. Plants inverted in the medium elongated and proliferated readily. Branching was obtained primarily from axillary buds several nodes basal to the apex. Gelling agent type did not affect proliferation. Vitrification was significantly affected by type of gelling agent, gelling agent concentration and thidiazuron concentration. In vitro shoots rooted readily even in medium containing adenine. Greater than 95% of the in vitro plants established in the greenhouse.

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