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The main goal of this research was to develop Campanula glaumerata `Acaulis' plants transformed with the isopentenyl transferase (ipt) gene for increased growth of the axillary buds and en-hanced insect resistance. Isopentenyl transferase is a first enzyme in the cytokinin biosynthetic pathway. For Campanula transformation, leaf discs were co-cultivated with Agrobacterium tumefacience LBA4404, which harbored the binary vector pBC34 (A. Smigocki, Beltsville, Md.) that codes for the nos-nptII gene and the ipt gene controlled by the CaMV35S promoter. The transformation frequency was about three times higher when leaf blade explants were infected with LBA4404 containing pBC34 as compared to infection with pGUSINT, which contains the gusint gene instead of the ipt gene. This difference in transformation frequency was attributed to expression of cytokinin from the ipt gene. Transgenic plant lines containing the ipt gene were verified by southern hybridization and divided into three groups by phenotype following culture in vitro on MS medium: 1) yellow/large leaves, no rooting; 2) green/large leaves, no rooting; 3) green/normal leaf size, rooting. These different phenotypes could be due to different levels of cytokinin expression in the transgenic plants.