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Tall fescue is an important cool-season grass widely used for forage and turf, and its genotypic variation for morphological traits has not been well documented. One hundred and fifteen tall fescue accessions, including 25 commercial cultivars, were divided into five groups based on their origination. The morphological traits, including plant height, spike length, pulvinus distance, spikelet count, branch count per spike, spike count per plant, and spike weight in different accessions were determined under field conditions in 2013 and 2014. There was significant genotypic variation in morphological traits among the 115 tall fescue accessions. Wild accessions exhibited a greater variation in the morphological traits than commercial cultivars. Close correlations were found among plant height, spike length, pulvinus distance, and spikelet count. The results of this suggest plant height, spike length, pulvinus distance, and spikelet count could be used as key morphological traits for evaluating all fescue germplasm effectively.