Garden impatiens (Impatiens walleriana), a very important floricultural crop in the United States, has been devastated by impatiens downy mildew (IDM) in recent years. This study was conducted to determine if induced tetraploidy could improve impatiens resistance to downy mildew. Tetraploids were induced by colchicine and confirmed by chromosome counting. Compared with diploids, induced tetraploids showed significant morphological changes, including larger and thicker leaves with larger and fewer stomata; thicker and fewer stems; larger and fewer flowers; and larger pollen grains with higher stainability. In detached leaf and in vivo inoculation assays, tetraploids exhibited improved downy mildew resistance, with lower disease severity, disease incidences, and sporangia densities. Plasmopara obducens, the causal agent of IDM, underwent a similar development process in the leaf tissue of diploids and tetraploids. These results suggest that induced tetraploidy can result in significant changes in impatiens leaf and plant morphology and can increase impatiens resistance to downy mildew to a certain extent.
Weining Wang, Yanhong He, Zhe Cao and Zhanao Deng
Zhi Quan, Bin Huang, Caiyan Lu, Yi Shi, Yanhong Cao, Yongzhuang Wang, Chuanrui He, Guangyu Chi, Jian Ma and Xin Chen
Much nitrogen (N) is lost in high-input protected cropping systems mainly via leaching of not only nitrate-N but also extractable organic N (EON), but the role of EON in this process is poorly appreciated. A consecutive 3-year plot experiment was conducted to investigate the impact of co-application of manures with chemical N fertilizer on N accumulation and loss in a greenhouse soil rotationally planted with cucumber or tomato and lettuce. Application of manures significantly enhanced the average contents and stocks of NO3 −-N, EON, and total N (TN) in 0- to 60-cm soil layer, although EON accumulated within growing season, while NO3 −-N accumulated with fluctuation, and TN accumulated gradually throughout the 3-year experiment. With application rate at 120 or 180 t dry manures per hectare per 3 years, the corresponding apparent N surplus was 2710 or 3924 kg⋅ha−1 per 3 years. Due to little increase of biomass N uptakes during vegetable seasons, the accumulated N in soil profile would be a potential loss source, largely via leaching of both nitrate and EON. Application of manures slowed soil acidification but intensified secondary salinization of the greenhouse soil. Considering the manures-induced high soil N accumulation and loss, well-balanced evaluation of the role of manures in high-input agricultural ecosystems is needed.