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- Author or Editor: Y. Sagawa x
Clonal propagation of ginger (Zingiber officinale Roscoe) was achieved by in vitro culture using buds from rhizomes in storage on a medium consisting of Murashige-Skoog major elements, Ringe-Nitsch minor elements and vitamins, 2% sucrose, and 1 ppm 6-benzylaminopurine (BA). Numerous adventitious shoots with roots were produced by repeated subculture of individual plantlets on 1 ppm BA medium. Rooted plantlets were successfully transferred to a mixture of 2 peat:1 sponge rock:1 vermiculite in the greenhouse and eventually to full sun in the nursery.
Clonal propagation in vitro of Aranda Wendy Scott ‘Greenfield’ and Aranthera James Storei was achieved with Vacin and Went medium (VW) supplemented with coconut water (CW) (15% by volume) or green banana (GB) (50 g/liter) or by omission of sucrose (Su). Induction of protocorm-like bodies (plb) on explants of apical and axillary buds was accomplished on VW + 15%CW and multiplication of plb in VW − 2%Su + 15%CW. Shoots were induced on solid VW − 2%Su for Aranthera James Storei and solid VW − 2%Su + 15%CW + 5%GB for Aranda Wendy Scott. Roots were induced on solid VW + 15%CW + 5%GB medium.
Shoots were produced in vitro from callus induced on stem expiants of Dracaena marginata ‘Tricolor’ on medium with Murashige and Skoog mineral salts, coconut water (15% by vol), supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and subcultured on medium supplemented with kinetin. Roots were obtained by subculture on medium supplemented with 1 mg/liter naphthaleneacetic acid (NAA). Rooted plantlets were transferred to potting medium in the greenhouse and subsequently transplanted to the field for further observation. The leaves of all derived plants lost the creamish-white stripe and resemble those of D. marginata, indicating D. marginata ‘Tricolor’ is a chimera.