Phomopsis cucurbitae is a latent infecting pathogen that infects unripe muskmelon fruit, but causes decay after harvest. This fungus causes severe losses during muskmelon fruit storage and marketing in the U.S., Japan, and some Central American countries. Previous studies showed that the fungus produced the cell wall-degrading enzyme polygalacturonase (PG) in both culture and muskmelon fruit tissue. The role of P. cucurbitae PG in the fruit decay process and its relation to latent infection is not well-understood. A prominent PG isozyme produced by the fungus in decayed fruit was purified to homogeneity by a sequence of extraction, ultrafiltration, preparative isoelectric focusing, anion exchange, and gel filtration chromatography. This isozyme exhibited endo-activity, a molecular weight of 54 kDa according to SDS-PAGE, and a pI of 4.2 based on IEF-PAGE. Isozyme activity was optimal at 40–45°C and pH 5.0. It had a Km of 44.7 g/ml and a Vmax of 0.313. The purified isozyme also effectively macerated mature muskmelon fruit tissues. This isozyme was the most prominent of the PG isozymes produced by P. cucurbitae in decaying fruit, and may play an important role in postharvest decay.
J.X. Zhang, B.D. Bruton and C.L. Biles
G.C. Munshaw, X. Zhang and E.H. Ervin
Bermudagrass [Cynodon dactylon (L.) Pers.] is widely used along its northern limit of adaptation. However, cold hardiness and winter survival are common concerns facing turfgrass managers in these areas. The objective of this study was to determine the effects of moderate salinity applications on bermudagrass cold hardiness. Two trials were conducted in Summer 2002. The cultivar Princess was seeded into pots in a glasshouse at a rate of 24 kg·ha-1. Pots received a weekly solution of 20-20-20 at a rate of 4.9 kg·ha-1 N. Bi-weekly salinity treatments began ≈2 months after germination and consisted of 0, 5, 20, and 40 dS·m-1 in the form of NaCl. These treatments continued for ≈8 weeks. Weekly quality ratings and chlorophyll fluorescence measurements showed similar results, with the high salinity treatments having the poorest quality. Soil electrical conductivity measurements showed a significant increase for the high salinity rates over the lower rates at the end of the trials. Proline concentrations increased with increasing salinity treatments in Trial 1 and were highest with the 20 dS·m-1 rate in Trial 2. Plants were acclimated in a growth chamber, and artificial freezing tests revealed that the 5 and 20 dS·m-1 treatments had the highest percentage of regrowth after freezing. These results indicate that moderate applications of salt or the use of effluent water prior to hardening may be an important way to increase bermudagrass cold hardiness.
X. Zhang, J.W. White and D.J. Beattie
Aquilegia × hybrida Sims `Purple' and `Dove' initiated flower buds 5 months after seeding without being exposed to low temperatures. Four experiments were conducted to test the effects of gibberellic acid (GA3), long photoperiod, long photoperiod with a high level of irradiance, and cold treatments on forcing of the two cultivars. Time from treatment to anthesis was reduced by 9 days for defoliated `Purple' plants treated with 250 mg GA3/liter, and by >14 days for defoliated `Dove' plants treated with 125 mg GA3/liter. Defoliated `Purple' or `Dove' plants treated with 18 hours of supplemental high pressure sodium (HPS) light at 250μl mol·m-1·s-2 (18 SH) reached anthesis 14 or 10 days earlier, respectively, after treatment than plants grown under natural daylight (Nat). The 18 SH treatment increased the number of flowers from eight and nine per plant on Nat plants for defoliated `Dove' and `Purple', respectively, to 16 flowers on 18 SH plants. Cold treatments at 4 ± lC did not shorten the interval between treatment and anthesis, but decreased the number of flowers per plant in both cultivars. Chemical name used: gibberellic acid (GA3).
X.E. Yang, X.Y. Lin, Y.S. Zhang and E.W. Stover
Citrus is a major fruit crop in the acid red soils (Ultisol) of hilly areas in Southeast China. These soils are normally deficient in P, K, and other elements. Integrated nutrient management is important for sustainable production of citrus in these areas. In this study, a systematic approach was used to identify the limiting factors for plant growth, using sorghum as a test species. Long-term field experiments were conducted with seven different P and K supply levels to determine optimal application rates for citrus (cv. Ponkan), following alleviation of other limiting factors. The primary nutritional limitations to plant growth in red acid soils included: severe deficiencies in N, P, and K, and moderate deficiencies in Ca, Zn, and B. With increasing application of P and K to field soil, N concentrations in citrus leaves decreased up to 60% due to dilution from increased growth, whereas P and K concentrations increased 2-3 fold. After 2 years of fertilizer application, the N: P: K ratio in leaves reached 1:0.5:1 for the optimal P and K treatment. The available P and K in the soils, measured after harvest each year, increased with increasing P and K application rates. However, within each treatment, increase in P and K with additional years of fertilization was modest. Citrus fruit yields generally increased with increasing P and K and reached a maximum at P and K rates of 125 kg P2O5/ha and 500 kg K2O/ha. In 3 years of successive field experiments, the highest net income was obtained by a balanced NPK fertilization practice using N: P2O5: K2O input of 450: 125: 500 kg/ha per year.
X.P. Zhang, B.B. Rhodes, W.V. Baird, H.T. Skorupska and W.C. Bridges
Hybrid seed production can be facilitated by using male sterility coupled with a seedling marker. This research was initiated to combine the ms male sterility and dg delayed-green seedling marker into watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] lines. Male-sterile plants of the male-sterile line G17AB were crossed with plants of delayed-green breeding line Pale90, which has yellow cotyledons and pale-green, newly developed, true leaves. The double-recessive recombinants, male sterile and delayed green, from the F2 population were backcrossed to the male-fertile plants of G17AB. The pedigree method was used for selection in the progenies. The segregation ratios obtained from F2 and BC1F2 populations suggest that the male-sterile and delayed-green traits are inherited independently and that delayed green is inherited as a single recessive nuclear gene. Two male-sterile watermelon lines with delayed-green seedling marker have been developed. These lines will provide a convenient way to introduce male sterility and the delayed-green seedling marker into various genetic backgrounds. These two lines can be used for testing the efficiency of a new, hybrid, watermelon, seed production system.
X. Zhang, F.M. Woods, R.C. Ebel, D.G. Himelrick, N.K. Singh and C. Mosjidis
A prevailing hypothesis indicates that a decrease in vegetative growth and cessation in floral initiation in strawberry in response to changes in photoperiod and temperature may correlate with hormonally induced changes. We investigated changes in endogenous free polyamines in crowns, flowers, leaves, and fruit of springbearing strawberries (Fragaria xananassa cvs. Chandler and Earliglow) in response to varying temperatures to induce flowering. Spermidine was the prominent free amine in crowns. No marked changes of putrescine, spermidine, and spermine were observed in crowns during the transition from vegetative to reproductive growth in either cultivar. In contrast, high levels of free polyamines were noted in young developing tissues such as the most recently initiated leaves, flower buds, and green fruit. When the putrescine synthesis inhibitor difluoromethylonithine (DFMO, 1 mm) was exogenously applied, levels of putrescine, spermidine, and spermine were altered in crown tissues in greenhouse experiments. These findings indicate that free polyamines may potentially be associated with the stimulation of new growth in springbearing strawberries under the present experimental conditions evaluated.
J.W. White, H. Chen, X. Zhang, D.J. Beattie and H. Grossman
Floral initiation and development of greenhouse and growth room-grown Aquilegia × hybrida Sims cultivars were studied using a scanning electron microscope. All greenhouse-grown cultivars initiated floral buds before cold treatment, ≈ 5 months after sowing. Floral initiation occurred at the apical meristem and proceeded acropetally on an elongated conical axis in the sequence: sepals, petals, stamens, stamenodia, and carpels. In a second experiment, 13 Aquilegia cultivars, three of which had been used in the first experiment, were grown as seedlings in a growth room at 20C under an 8-, 12-, 16-, or 20-hr photoperiod, each totaling 10.2 mol·day-1·m-2 irradiance from cool-white fluorescent lamps. Here, floral initiation was absent even after 7 months from sowing, presumably because there was no diurnal variation in irradiance or temperature.
X.P. Zhang, B.B. Rhodes, W.V. Baird, W.C. Bridges and H.T. Skorupska
This research was conducted to develop genic male-sterile lines of watermelon (Citrullus lanatus Matsum & Nakai) homozygous for the juvenile albino (ja) seedling marker. Male-sterile plants (msms) of the genic male-sterile line G17AB were crossed with a Dixielee plant that was heterozygous for the ja locus. Male-fertile, juvenile albino recombinants of the F2 progeny were self-pollinated, resulting in F3 progeny. The male-sterile normal green recombinants of the F2 progeny were crossed with an F1 hybrid plant with genotype MsmsJaja, and three populations (93JMSB-1, -2, and -3) were obtained from these crosses. Juvenile albino recombinants were confined to 93JMSB-1. Of the juvenile albino plants of 93JMSB-1, male-sterile plants were sib-crossed with male-fertile plants, resulting in 93JMSB-1-1. Progeny of 93JMSB-1-1 was homozygous for ja and segregated for ms in a 127 male-sterile: 128 male-fertile ratio, fitting a 1:1 ratio. The male-sterile juvenile albino plants of F3 were crossed with male-fertile juvenile albino plants of 93JMSB-1, resulting in 93JMSF3-1 and -2. Plants 93JMSF3-1 and -2 were homozygous for ja but segregated for ms at 10 male-sterile: 13 male-fertile and 15 male-sterile: 19 male-fertile for 93JMSF3-1 and 93JMSF3-2, respectively, fitting the 1:1 ratio. These three genic male-sterile lines with the ja seedling marker provide valuable germplasm for introducing ms and ja genes into diverse genetic backgrounds and for studying cross-pollination and gene flow in watermelon populations.
Y.C. Sun, Y.J. Zhang, K. Wang and X.J. Qiu
Iris lactea seed is characterized mainly by physiological dormancy. Two experiments were conducted to investigate the effect of NaOH treatment and stratification on Iris seed germination. In Experiment 1, seeds were treated with 14.38 m NaOH for 0 to 28 hours. In Experiment 2, NaOH treated and nontreated seeds were stratified under 7 °C and moistened condition for 0 to 40 days. As results, NaOH treatment for 20 hours effectively removed seedcoat and improved germination percentage from 0% to 56% compared to control (0 hours). However, germination percentage started to decrease after 20 hours. Stratification for 40 days further improved germination percentage of NaOH treated seeds to >80%, but did not affected seeds without NaOH treatment. Results demonstrate that combination of NaOH treatment and stratification is an effective practice to break Iris seed dormancy and improve germination percentage.
X. Zhang, B.B. Rhodes, W.V. Baird, H.T. Skorupska and W.C. Bridges
juvenile albino (ja) is a spontaneous mutant, first observed in 1992. Hypocotyls, new young leaves, shoot tips, tendrils, and flowers on the main shoot of the ja mutant are all albino during early spring and late fall. The interior of the albino leaves gradually become green, while the margins remain albino. Fruit rind color of the mutant is variegated. Growth of the ja mutant is severely impaired in the early spring and late fall. However, the mutant grows almost normal in the summer, and produces fruits of almost normal size. Genetic analysis of F1, F2, and BC1 populations derived from the ja mutant showed that ja mutant is inherited as a single, recessive, nuclear gene. The segregation ratios in the F2 and BC1 progenies derived from the cross between the previously reported dg virescent mutant and the ja mutant indicated that both are inherited independently. Experiments with temperature (3–5C vs. 20–22C at night), day length (8 vs. 15 h), and red and/or far-red light (15 vs. 0 min) at the end of an 8-h day were performed to investigate the regulation of ja trait expression. Temperature and red/far-red light had no differential effect on mutant and wild-type plants. However, significantly increased fresh weight and chlorophyll content were observed in the ja mutant over the wild-type when grown under long-day conditions. In addition, chlorophyll synthesis or accumulation in the mutant is severely impaired under short-day conditions. To our knowledge, this is the only virescent mutant in Cucurbitaceae whose expression is regulated by day length.