The sugar content of the sweetpotato cultivars Centennial, Coroner, Georgia Red, Jewel, and Sweet Red was measured by nign performance liquid chromatography (HPLC) and compared to the sugar content found by measuring the refractive index of cellular sap and converting the refractive index value to sugar concentration. The sugar content and refractive index values were measured for just-harvested, cured and stored roots. Changes in the sugar content as determined by refractive index were found to be linearly related to changes 1n sugar content measured by HPLC, indicating that this method can be used to monitor changes in postharvest sugar content.
william M. Walter Jr.
William M. Walter Jr.
The sugar content of five sweetpotato [Ipomoea batatas (L.) Lam.] cultivars (`Centennial', `Cordner', `Georgia Red', `Jewel', and `Sweet Red') was measured by high-performance liquid chromatography (HPLC) and compared to the sugar content of the cellular sap measured by refractive index (RI). The HPLC and RI sugar contents were measured at harvest, after curing, and during storage. Changes in the sugar content, as determined by the RI, were found to be linearly related to changes in the sugar content of cell sap and tissue, as measured by HPLC, indicating that this method can be used to monitor changes in postharvest total sugar content.
William M. Walter Jr., Betsy Randall-Schadel, and William E. Schadel
Wound healing in cucumber fruit (Cucumis sativus L., cv. Calypso) was studied using histological and degradative techniques. A thick exudate appeared at the wounded surface shortly after wounding. This material retarded water loss and possibly aided in the formation of sclerified parenchyma observed 24 hours after wounding. The sclerified material was positive to a modified Weisner stain, indicating lignification was occurring. Wound periderm (cork) was initiated directly beneath the sclerified parenchyma cells within 48 hours after wounding. The cork layers were positive to Sudan IV stain, indicating suberin was being formed. The rate of phellem development decreased by 6 days after wounding. By day 7, younger phellem cells and sclerified parenchyma cells were stained by Sudan IV. Degradation of the wound tissue by chemical procedures demonstrated that relatively large amounts of lignin and suberin were deposited during healing. Fragments from the lignin degradation Indicated that lignin was composed mainly of gualacyl and p-hydroxyphenyl residues. Suberin was found to contain mainly 1,16-hexadecane and 1,18-osctadecene decarboxylic acids detected as the silylated diol derivatives.