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Free access

Wei Zheng, Xiao-dan Xu, Kai-Ge Zhao, and Longqing Chen

Free access

Yihui Cui, Peng Zhao, Hongqiang An, Nan Lv, Zifeng Zhang, Wei Pei, and Wanjun Wang

To find the characteristics of somatic embryogenesis of orchids and elucidate the mechanism, we had previously established an efficient plant regeneration system via somatic embryogenesis in Dendrobium candidum Wall ex Lindl. In this study, a detailed cytological investigation was carried out on the initiation and developmental process of somatic embryogenesis. Based on our observations, the somatic embryogenesis in D. candidum originated from the transition of an embryonic callus cell to the initial somatic embryo cell, and the somatic embryos initiated from those cells. During the transition process, condensation and devacuolation successively occurred in the cytoplasm of the embryonic callus cells, giving rise to the formation of a typical initial somatic embryo cell with dense cytoplasm and a clear nucleus. One of the two pathways in somatic embryogenesis is the single-cell-derived somatic embryo which is generated from an inner initial somatic embryo cell in embryonic callus and develops into a globular somatic embryo in a way similar to zygotic embryogenesis and then keeps developing into a protocorm-like body (PLB). The other is a multiple-cell-derived somatic embryo which is generated from peripheral grouped initial somatic cells in embryonic calli and directly forms globular embryo or multicellular somatic proembryo, lacking the typical early stages of embryogenesis. Both pathways were observed in the somatic embryogenesis system, indicating that the culture system in D. candidum can be a useful tool for investigating the mechanisms underlying orchid embryogenesis.

Full access

Haiyan Zhao, Haiying Liang, Yibing Chu, Congcong Sun, Ning Wei, Mengnan Yang, and Caixia Zheng

Ginkgo biloba L. (ginkgo) is generally regarded as a tolerant species to environmental stresses. However, its tolerance mechanisms are not well understood, particularly for salt stress. To evaluate the species’ physiological responses to salt stress, 3-year-old ginkgo seedlings were exposed to a range of salinity levels (0% to 1.0% NaCl). A significant reduction in maximum (F v/F m) and actual (ΦPSII) quantum yields of photosystem II (PSII) photochemistry and the nonphotochemical quenching (qN) coefficient only occurred in late treatment stages at the salinity levels of 0.6% to 1.0%. As salt concentration increased, the response time and chlorophyll (Chl) fluorescence indices decreased. Overall, the activities of superoxide dismutase (SOD) and peroxidase (POD); contents of catalase (CAT), reduced glutathione (GSH), and flavonoids; and scavenging rate of free radicals enhanced under salinity stress. These data indicate that ginkgo seedlings are tolerant to low salt stress, and enzymatic and nonenzymatic antioxidant systems seem to work synergistically to reduce lipid oxidation under NaCl stress because malondialdehyde (MDA) content did not increase. Correlation and principal component analyses determined that water potential, Chl fluorescence parameters, activities of POD and SOD, contents of CAT and flavonoids, and hydroxyl (•OH) and diphenyl picrylhydrazyl (DPPH) free radical scavenging capability were sensitive to salt stress. These parameters can be used for in vitro or rapid and nondestructive monitoring of the responses of ginkgo seedlings to salinity stress. It is of significance to understand the tolerance mechanisms of ginkgo to salt stress, reduce the harm of NaCl and other snow-melting agents to ginkgo as shade trees, and develop new salt-tolerant varieties.

Open access

Jingjing Kou, Zhihui Zhao, Wenjiang Wang, Chuangqi Wei, Junfeng Guan, and Christopher Ference

‘Mopan’ persimmon (Diospyros kaki Thunb.) is a traditional astringent cultivar of persimmon and ‘Yoho’ persimmon (D. kaki) is a newly introduced Japanese nonastringent type of cultivar in northern China. Studies were conducted to investigate the physiological changes and expression of ripening-related genes in the postharvest process at different periods under the effects of endogenous ethylene in both cultivars. Persimmons were harvested and stored under room temperature for 20 days. An analysis of physiological changes showed significant differences between the two cultivars. Total soluble solids declined in ‘Mopan’ fruit, whereas those in ‘Yoho’ fruit increased during storage. Firmness, color, index of absorbance difference, total and soluble tannin contents, ethylene production, and respiration rates showed the same trend, but these values vary by cultivar. ‘Mopan’ fruit softened rapidly after harvest and attained edible quality in 20 days, with an increased rate of softening accompanied by increased expression of ripening-related genes. In contract, ‘Yoho’ fruit softening occurred slowly and did not soften even after 20 days, with minimal accumulation of the ripening-related genes. The information obtained from this study demonstrates that cell wall-hydrolyzing enzymes, the de-astringent process, and endogenous ethylene have critical roles in postharvest ripening, gene expression, and physiological property changes of ‘Mopan’ and ‘Yoho’ persimmon fruit during storage.

Free access

Xin Hao, Yu Fu, Wei Zhao, Lifei Liu, Rengui Bade, Agula Hasi, and Jinfeng Hao

The MADS-box gene family encodes a type of transcription factor, and plays a key role in the growth and development of plants. Here, we identified 62 MADS-box genes in the melon (Cucumis melo) genome using bioinformatics methods. These genes were divided into type I Mα, Mγ, and Mδ subfamilies (26 members) and type II MIKCC subfamilies (36 members) by phylogenetic analysis. There were no genes in type II AGL12, BS, TM8, and MIKC* subfamilies, and type I Mβ subfamilies. Conserved motif analysis showed that all motifs had a subfamily-specific distribution except the M domain. The expression analysis of the MADS-box genes showed different expression characteristics. In summary, this study is the first to identify melon MADS-box genes and analyze their gene structures, subfamily distribution, and expression characteristics. These results provide a foundation for investigating the functions of the melon MADS-box genes.

Open access

En-chao Liu, Li-fang Niu, Yang Yi, Li-mei Wang, You-wei Ai, Yun Zhao, Hong-xun Wang, and Ting Min

Ethylene response factor (ERF) genes have been characterized in numerous plants, where they are associated with responses to biotic and abiotic stress. Modified atmosphere packaging (MAP) is an effective treatment to prevent lotus root browning. However, the possible relationship between ERF transcription factors and lotus root browning under MAP remains unexplored. In this study, the effects of phenol, phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) enzyme activities; and PPO, PAL, POD, and ERF gene expression on fresh-cut lotus root browning were studied with MAP. The expression pattern of ERF2/5 correlated highly with the degree of browning. It is suggested that NnERF2/5 can be used as an important candidate gene for the regulation of fresh-cut lotus root browning under MAP, and the correlation of each gene should be studied further.

Free access

Anne Plotto, Elizabeth Baldwin, Jinhe Bai, John Manthey, Smita Raithore, Sophie Deterre, Wei Zhao, Cecilia do Nascimento Nunes, Philip A. Stansly, and James A. Tansey

A 3-year study was undertaken to establish the effect of field nutritional sprays, combined with insecticide treatments or not against Asian Citrus psyllid, on the fruit quality of ‘Valencia’ orange trees affected by the greening disease Huanglongbing (HLB). Four replicated plots were harvested, juiced, and pasteurized. Nine to twelve trained panelists evaluated the juice using seven flavor, five taste, four mouthfeel and three aftertaste descriptors. There was little difference between treatments in 2013; only orange peel flavor and bitterness were significantly lower for the insecticide treatment. In 2014, positive attributes, such as orange and fruity flavor, sweetness and mouthfeel body, were significantly higher in the insecticide treatment. Sourness was highest in untreated control, and there were no differences between treatments for bitterness. In 2015, negative attributes, such as grapefruit, orange peel and typical HLB flavor, sourness, bitterness, and astringency, were significantly higher in untreated control fruit, suggesting perhaps that the beneficial effect of nutritional and insecticide treatments was cumulative, only manifesting on the 3rd year of the study, and or because of the progression of the disease affecting untreated controls. Data are discussed in relation to juice chemical composition, including volatiles, sugars, acids, limonoids, and flavonoids, adding to the fundamental knowledge concerning chemical drivers of orange flavor.

Free access

Elizabeth Baldwin, Jinhe Bai, Anne Plotto, John Manthey, Smita Raithore, Sophie Deterre, Wei Zhao, Cecilia do Nascimento Nunes, Philip A. Stansly, and James A. Tansey

‘Valencia’ orange trees from groves with 90% infection by Candidatus liberibacter asiaticus (CLas), the presumed pathogen for citrus greening or huanglongbing (HLB) disease, were treated with insecticide (I), a nutritional spray (N), and insecticide plus nutritional spray (I + N). Controls (C) were not treated. Fruit were harvested in March to April, 2013, 2014, and 2015, juiced, and the juice was frozen for later chemical analyses. Titratable acidity (TA), soluble solids content (SSC), SSC/TA ratio, many volatiles, flavonoids, and limonoids showed differences because of season, whereas SSC, several volatiles (ethanol, cis-3 hexenol, α-terpinene, ethyl acetate, and acetone), flavonoids (narirutin, vicenin-2, diosmin, nobiletin, heptamethoxy flavone), and limonoids (nomilin and nomilinic acid glucoside) showed differences because of treatment. However, consistent patterns for chemical differences among seasons were not detected. TA tended to be higher in N and C the first two seasons and SSC/TA higher in I and I + N for all seasons (not significant for 2014). Bitter limonoids tended to be higher in I, N or I + N over the seasons. Principal Component Analysis showed that there was a good separation by season overall and for treatment in 2013. In 2014 and 2015, the insecticide treatments (either I or I + N) had the highest sugar and SSC/TA levels and lowest TA levels, although not always significant, as well as higher juice CLas cycle threshold (Ct) levels, indicating lower levels of the pathogen.