Progenies and clones of interspecific hybrid blueberries were evaluated for annual fraction of canopy volume (FCYV) and for difference in fraction of canopy volume between control and stressed plants [FCYV(C) - FCYV(S)] in a moderate water-deficit environment. The FCYV(C) - FCYV(S) data were used to determine combining ability effects. In addition, physiological processes of attached leaves of the clones were monitored with a portable photosynthesis apparatus. Specific combining ability (SCA) effects were significant for FCYV(C) - FCYV(S). The clone with the lowest mean for FCYV(C) - FCYV(S) was US75, a hybrid of Vaccinium darrowi Camp × V. corymbosum L. Clone JU64 (V. myrsinites Lamark × V. angustifolium Aiton) also had a low FCYV(C) - FCYV(S) mean, and its two progenies (JU64 × JU11 and G362 × JU64) had low progeny means. Stomatal conductance was lowered when blueberries were exposed to atmospheric and/or soil moisture stress that resulted in lower transpiration and photosynthesis and increased or equal water-use efficiencies (WUE). Blueberry plants adjusted to moisture stress as the season progressed by lowering stomatal conductance and increasing WUE. In particular, stressed plants of US75 and JU64 had equal or higher WUE values than control plants. US226 was the most drought-susceptible clone in the study, and its stomata did not appear to be as responsive to moisture stress as the other clones. Breeding for higher WUE in a dry environment appears possible with the germplasm used in this study.
W.A. Erb, A.D. Draper, and H.J. Swartz
W.A. Erb, A.D. Draper, and H. J. Swartz
The relationship between moisture stress and mineral soil tolerance was studied by placing 10 blueberry (Vaccinium) clones in a Berryland sand soil high in organic matter (Berryland) and a Galestown sandy clay loam soil (Galestown) and subjecting them to one of two moisture regimes. The Berryland and Galestown soils represent an excellent blueberry soil and a mineral soil, respectively. A moderate degree of water stress influenced biomass partitioning in blueberries in a similar manner as stress induced by culture on mineral soil. Berryland control plants on Berryland partitioned more biomass into leaves and produced more dry matter and leaf area than plants on Galestown or those moisture stressed. Net assimilation rate and relative growth rate were not significantly different between soil or moisture treatments. The primary reason for the reduction in absolute growth rate due to soil type or moisture stress was a significantly lower leaf area duration on Galestown soil and in-moisture stressed plants. Clones differed in instantaneous transpiration, leaf conductance, and apparent photosynthesis and the ability to partition biomass into various plant parts. By selecting for increased leafiness, a high photosynthetic rate, and a more energy efficient root system, improvement in mineral soil tolerance should be possible.
W.A. Erb, A.D. Draper, and H.J. Swartz
Interspecific blueberry (Vaccinium spp.) progenies were examined to determine combining abilities and genetic variability for seedling root system size and shoot vigor and to establish whether a large root system is correlated with good growth when plants are grown on a mineral soil and exposed to a moderate soil water deficit. General combining ability (GCA) variance components for root system size and shoot vigor and specific combining ability variance components for shoot vigor were significant. US226, a tetraploid hybrid of V. myrtilloides Michaux × V. atrococcum Heller, had the highest GCA effect for root system size and the lowest GCA effect for shoot vigor. US75 (V. darrowi Camp × V. corymbosum L.) had the highest GCA effect for shoot vigor and was second in GCA effect for root system size. Comparison of the crosses containing G111 (V. corymbosum) with those containing G362 (V. corymbosum) indicates that selecting for the best V. corymbosum clone to start a breeding program seems as important as selecting the mineral soil-adapted parent. Root system ratings were highly correlated with total dry weight of field-grown plants (r = 0.89). The method used in this study to evaluate seedlings for root system size and shoot vigor could be used to eliminate the less vigorous plants from a population before field planting and to evaluate mineral soil adaptability.
W.A. Erb, A.D. Draper, G.J. Galletta, and H.J. Swartz
Data from a four-parent diallel, involving one highbush (Vaccinium corymbosum L.) clone and three interspecific hybrids grown on mineral soil unamended with organic matter, were analyzed to determine combining ability effects for six traits: plant size, berry size, the number of days between flowering and fruiting (# DBF&F), the ratio of total fruit weight to canopy volume (TFW: CYV), days to fruit ripe, and yield. General combining ability effects were significant for all characters tested, except yield and berry size in 1984. Specific combining ability effects were significant for plant size in 1983, #DBF&F in 1984, TFW: CYV in 1984, and berry size in 1985. Vigorous and productive highbush cultivars can be developed for mineral soils by using the interspecific clones from this study and their selected recombinant to combine the genes for plant vigor with the high-quality fruit traits of highbush cultivars.
W. A. Erb, A. D. Draper, and H. J. Swartz
Two methods of evaluating seedling drought resistance in Vaccinium (blueberry) spp. were examined. Twenty interspecific populations were greenhouse-grown and either matric-stressed in a dry 1 sand : 1 soil medium or osmotic-stressed in a nutrient solution containing polyethylene glycol (PEG). In both tests, population means were separated statistically by shoot damage ratings. The correlation (r = 0.46) between the two tests was positive and significant. Progenies of clones JU64 and JU62, which are sister seedlings (V. myrsinites Lamark × V. angustifolum Aiton), were the most drought-resistant. The soil screening test appeared more accurate because it grouped populations with common parentage. These tests indicated that the progenies differ in genetic capacity to resist drought.
L. Mark Lagrimini, Jill Vaughn, W. Alan Erb, and Sally A. Miller
Lignin composition in leaf, fruit, and fruit outer epidermis of transgenic tomato (Lycopersicon esculentum Mill.) plants that overproduce the enzyme tobacco anionic peroxidase (TobAnPOD) was analyzed. This enzyme may catalyze the polymerization of cinnamyl alcohols into lignin in tobacco (Nicotiana tabacum L.); therefore, we predicted that its presence in the transformed tissue would increase lignin levels in healthy and wounded tissue. Lignin levels in healthy plants increased by 20% in leaf, 49% in fruit, and 106% in fruit outer epidermal tissue. Mature-green fruit were aseptically wounded and incubated in darkness for up to 7 days. Soluble phenols in wounded transgenic fruit increased by more than 300% hut changed little in control fruit. As with soluble phenols, lignin content in wounded transformed fruit increased by more than 20-fold hut increased less than two-fold in control fruit. Transgenic seedlings overproducing TobAnPOD were screened for susceptibility to several pathogens, but resistance did not increase. Possible TobAnPOD roles in lignin biosynthesis, phenol metabolism, stress response, and disease resistance are discussed.
W. A. Erb, J. N. Moore, and R. E. Sterne
The attraction of zoospores of Phytophthora cinnamomi Rands to roots of three cultivars of rabbiteye blueberry (Vaccinium ashei Reade), two species hybrid cultivars of highbush blueberry, and one tetraploid species hybrid selection (US 109) was compared. Zoospores were attracted to the roots of all plants tested. Roots of highbush cultivars ‘Bluetta’ and ‘Patriot’ attracted more zoospores than the rabbiteye cultivars. The number of zoospores attracted to roots of US 109 was greater than the number attracted to the three rabbiteye cultivars, but less than the highbush cultivars. Increased zoospore attraction appeared to be related to root rot susceptibility in blueberries.