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  • Author or Editor: Vincent Fritz x
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Turnips (Brassica rapa. subsp. rapa L.) produce glucosinolates (GSLs), thioglucosides whose hydrolyzed derivatives have been shown to provide chemopreventive benefits. Two cultivars of turnips [‘Just Right’ (JR) and ‘Scarlet Queen’ (SQ)] were grown under three different temperature regimes to assess the role of temperature on GSL production in roots and shoots. When compared with low-temperature treatments, high-temperature treatments increased total and individual GSLs in a tissue- and genotype-specific manner. When compared with low-temperature treatments, total GSLs were ≈70% and 130% higher in JR shoots and roots, respectively, grown at high-temperature treatments. High temperatures also increased total GSLs in SQ shoots and roots by ≈80% and 85%, respectively, when compared with low temperatures. Gluconasturtiin (GNS, 2-phenylethyl GSL) concentration was inversely correlated with temperature with high-temperature treatments resulting in 20% and 48% less GNS than low-temperature treatments in JR and SQ roots, respectively. The indolic GSL, 1-methoxyglucobrassicin (1MGB; 1-methoxy-3-ylmethyl GSL), was the root GSL most elevated by increased temperature resulting in a 1000% increase on average in both cultivars between the low- and high-temperature treatments. These results show promise for the use of temperature to enhance the health-promoting properties of turnip because 1MGB has potent chemopreventive effects. Gene expression analysis suggests that some BrMYB transcription factor expression levels are associated with temperature-dependent changes in GSL accumulation; however, this association varies between cultivar and tissue type.

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Germination and stand establishment of shrunken-2 sweet corn is a major production-related problem, particularly during early spring in the upper Midwest U.S. Several potential physical and physiological factors have been identified as contributors to poor stand establishment. Poor pericarp integrity and high sucrose levels may lead to non-uniform moisturization and imbibitional injury. Studies were conducted to determine if the use of “controlled moisturization” preplant treatments reduced imbibitional injury and improved germination and seedling vigor. Both laboratory and field results will be discussed.

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It is commonly recommended to apply phosphorus- or nitrogen- and phosphorus-containing water-soluble fertilizers to annual vegetables at transplant to improve establishment and enhance yield. Plastic mulches are also recommended to increase soil temperature and enhance yield through similar root growth-promoting mechanisms early in the season. Our aim was to determine if the recommendations for transplant fertilizer solutions and plastic mulch are justified, and if the effects are interactive in a clay loam soil with moderate or high levels of existing phosphorus fertility and organic matter. We transplanted ‘Plum Dandy’ tomato (Solanum lycopersicum) in 2014 and 2015 into a field with high fertility using black polyethylene mulch or no mulch, and transplant solution containing water, 320 mg/plant nitrogen, or 320 mg/plant nitrogen + 475 mg/plant phosphorus. Mulch was removed 26 to 28 days after transplanting to eliminate midseason and late season mulch effects. We found yield-promoting and maturity-hastening effects in both years from transplant solutions containing both nitrogen and phosphorus (18% greater total ripe fruit weight than water control), and similar benefits of early season black plastic mulch (24% greater total ripe fruit weight than no mulch), indicating usefulness of either treatment in tomato production. We found no interactive effects of mulch and transplant solution.

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Gluconasturtiin (2-phenylethyl glucosinolate), an aromatic glucosinolate, was used to evaluate the response of Chinese cabbage (Brassica campestris L. ssp. pekinensis) cv. Green Rocket to three and five cabbage looper (Trichoplusia ni Hubner) larvae per plant. Plants were harvested 0, 10, and 17 days after infestation. The change in gluconasturtiin content due to decreased light and leaf area removed was also studied. All samples were assayed for gluconasturtiin content using high performance liquid chromatography (HPLC). The gluconasturtiin content of plants subjected to five larvae/plant had a 59% increase, compared to noninfested plants 10 days after infestation. The effect of larval feeding was also dependent on harvest time. The levels of gluconasturtiin increased by 52% from the first harvest (prior to infestation) to the second harvest (10 days after infestation) in both larval feeding densities. Seventeen days after infestation (final harvest), gluconasturtiin content experienced a nonsignificant 6% decrease, compared to the previous harvest.

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This study was conducted to determine if changes in the raffinose: sucrose ratio in embryos of shrunken-2 sweet corn (Zea mays L.) hybrids were related to differences in seed leachate conductivity between two hybrids harvested at four maturities and artificially dried to 0.10 g H2O/g fresh weight. The ratio of raffinose: sucrose differed for `Crisp N' Sweet 710' (CNS) and `How Sweet It Is' (HSII). The mass ratio of raffinose: sucrose in CNS was >0.3 in seed harvested between 0.44 to 0.64 g H2O/g fresh weight and increased as seed dried from the initial harvest moisture to 0.10 g H2O/g fresh weight. Raffinose: sucrose ratios of HSII were <0.3 at all harvests between 0.55 to 0.72 g H2O/g fresh weight, but changes during desiccation were not as pronounced. Leachate conductivity of whole seeds of CNS and HSII decreased as seeds were harvested at progressively lower moisture contents. We suggest that a higher raffinose: sucrose ratio may be indicative of increased seed vigor in shrunken-2 hybrids.

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Sweet corn silage waste is ≈18% dry matter and contains 1.2% N and 0.26% P on a dry-weight basis. Silage waste in rates of 0 to 448 T·ha–1 was applied to a previously harvested sweet corn field in late summer. Beginning the following spring, soil samples were periodically collected to follow the rate of N mineralization. Field corn was planted to the site the following spring as the test crop. At harvest, grain, stover, and silage yields were recorded and N removal from the system was followed through grain and tissue sampling. Additional studies were also conducted to evaluate the impact of primary tillage method on subsequent N mobilization from sweet corn silage waste and to assess the residual N release potential beyond the first year following silage waste application. Results suggest that land application of sweet corn silage waste at 224 T·ha–1 would be environmentally responsible, provided that adequate nitrogen credit from the silage waste is integrated into the total nitrogen needs of the subsequent crop. Greater mineralization is achieved when the silage waste is moldboard plowed compared to chisel plowing. Chisel plowing could result in greater residual N carryover during the year following silage waste application. Seedling emergence rates were faster and grain yield was superior in some years in moldboard-plowed plots compared to chisel-plowed plots. Further calibration of additional N fertilizer on land that received silage waste is necessary for improved production efficiency and sweet corn silage waste use in production systems.

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Sweet corn (Zea mays L. var. rugosa Bonaf.) seed carrying the mutant endosperm gene shrunken-2 (sh2) are very susceptible to seed rot and pre- and post-emergence damping off. Experiments were conducted to determine if selected organic solvents were suitable carriers for fungicide infusion of sh2 sweet corn seed for improved germination and stand establishment. Seed of `Florida Staysweet' and `Crisp-n-Sweet 710' were immersed in acetone, cyclohexane, decahydronaphthalene (Decalin), dimethylsulfoxide (DMSO), ethanol, or xylene for 5 seconds, 0.25, 0.50, 1.0, 2.0, 4.0, or 8.0 hours, air-dried, and subjected to a cold-stress test. Total germination and percentage of normal seedlings in both cultivars were significantly decreased after 8 hours of immersion in acetone. Average seedling dry weight, however, did not decrease. DMSO was highly toxic to both cultivars. Ethanol increased seed mortality with increasing immersion times. Cyclohexane, Decalin, and xylene caused erratic responses in all measured variables as immersion time increased. In a second experiment, the effects of immersion time up to 4 hours in acetone on germination and vigor of 11 sh2 cultivars were compared. There was no correlation between cultivar germination or vigor and immersion in acetone. Results indicate acetone could be used to infuse fungicides into the seed of some sh2 cultivars without compromising seed germination or vigor. However, each sh2 cultivar must be screened individually to determine if it is a suitable candidate for organic solvent infusion of fungicides.

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The objective of this study was to examine seed maturity at harvest as it relates to seed vigor in two commercial shrunken-2 (sh2 J sweet corn hybrids (Zea mays L. var rugosa Bonaf., cvs. Florida Staysweet, Crisp N' `Sweet 710). Seed harvest began at 0.76 g H2O/g fresh weight in 1987 and at 0.70 g H2O/g fresh weight in 1988 and 1989, and was continued at gradually declining moisture levels until frost. In five different tests of seed performance, seed of `Florida Staysweet' (FLASS) harvested between 0.23 to 0.57 g H2O/g fresh weight in 1987 possessed the highest seedling vigor. In 1988 and 1989, maximum vigor was achieved by FLASS seed harvested from 0.40 to 0.60 g H2O/g fresh weight and `Crisp N' Sweet 710' (CNS) seed harvested from 0.45 to 0.65 g H2O/g fresh weight. Standard germination test, seedling growth cold test (SGCT), and seed leachate conductivity provided the most consistent results to `determine optimum seed maturity. Seed weight was not as reliable an indicator of seed vigor in 1988 and 1989 as it was in 1987, and endosperm and embryo weights did not correlate with seedling vigor in any year.

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Abstract

Sand nutrient cultures (containing different Ca2+ compositions) were used to modify the petiole Ca2+ concentrations in Chinese cabbage (Brassica campestris Group pekinensis). Petiole sections (6.5 cm2) from inner and outer leaves, 30 and 60 days after transplanting, were inoculated with Erwinia carotovora ssp. carotovora to evaluate resistance to soft rot incidence and disease progression. Xylem exudates collected from the plants in the sand nutrient culture were analyzed for Ca2+ concentration. Elevated petiole Ca2+ concentrations were not correlated with the development of soft rot in inoculated petiole sections. Plant age and petiole position (inner and outer) did not consistently affect soft rot development. There was a trend of reduced soft rot in inoculated petiole (inner) sections of ‘Hakuran’ plants as the xylem exudate Ca2+ levels increased.

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