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- Author or Editor: Valerie Creaser Pence x
Abstract
Cultured immature sexual embryos of Theobroma cacao proliferated asexual embryos, but embryogenesis was not observed from leaf, pericarp, or ovule tissues. Cotyledons from immature, unpigmented embryos (2.5 to 10 mm long) were highly embryognie, while larger more mature, pigmented cotyledons initiated roots as their only form of organized development. Embryogenesis occurred in light and darkness when a basal medium was supplemented with coconut water and 1.5 mg/liter NAA. Proliferated embryos recultured in a liquid medium continued development.
Abstract
Cotyledonary development of asexual embryos of cacao (Theobroma cacao L.) in liquid medium on a rotary drum apparatus was similar to that of zygotic embryos in vivo. Immature zygotic and asexual embryos synthesized anthocyanins, characteristic of the developing zygotic embryo in vivo, when cultured in vitro in liquid or on semisolid media containing high concentrations of sucrose.