Search Results

You are looking at 1 - 2 of 2 items for

  • Author or Editor: Ting Min x
Clear All Modify Search
Restricted access

Ting Min, Jun Xie, Yang Yi, Wenfu Hou, Youwei Ai and Hongxun Wang

Ethylene response factor (ERF) genes have been characterized in numerous plants in which they are involved in responses to biotic and abiotic stress, including cold and heat stress. Cool temperatures is one of the most effective storage methods for delaying browning of fresh-cut lotus (Nelumbo nucifera) root. In model plants, ERF genes have been identified as being responsive to cold and heat stress. Whether ERF is associated with lotus root browning in cooler temperatures has not been studied. In this research, low-temperature storage (4 °C) effectively delayed browning of fresh-cut lotus root. Using RNA sequencing, seven Nelumbo nucifera ERF (NnERF) genes were isolated and studied. Transcriptional analysis indicated NnERF genes responded differently to temperature. NnERF3/4/5 were reduced continuously by a low temperature (4 °C) and NnERF5 was the most strongly downregulated. In contrast, transcripts of NnERF1/2/7 were increased at a low temperature (4 °C). The expression of NnERF6 showed no obvious difference between the two different temperatures. It is proposed that NnERF3/4/5 could be important candidates as regulators of fresh-cut lotus root browning. The roles of other members are also discussed.

Restricted access

Ting Min, En-chao Liu, Jun Xie, Yang Yi, Li-mei Wang, You-wei Ai and Hong-xun Wang

Ethylene response factor (ERF) genes have been involved in responses to biotic and abiotic stress, including hypoxia and anaerobic stress. Vacuum packaging (a typical anaerobic stress) is an effective storage method used to delay browning of fresh-cut lotus root (Nelumbo nucifera). In model plants, ERF genes have been identified as responsive to hypoxia. Whether ERF is associated with browning of vacuum-packaged lotus root has not been studied. The effects of vacuum packaging on browning, phenolic content, the enzyme activity of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD), and PPO, PAL, POD, and ERF genes expression in fresh-cut lotus root were studied. Downregulation of NnPAL1, NnPPOA, and NnPOD2/3 attributable to vacuum packaging coincided with increased related enzyme activities and the degree of browning of fresh-cut lotus root. The expression patterns of NnERF4/5 were consistent with the changes in NnPAL1, NnPPOA, and NnPOD2/3 gene expression. It has been proposed that NnERF4/5 could have be important regulators of fresh-cut lotus root browning, and that the relationships of NnERF4/5 and NnPAL1, NnPPOA, and NnPOD2/3 should to be studied further.