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Timothy F. Wenslaff and Paul M. Lyrene

Two clones of anthocyanin-deficient (AD) Vaccinium elliottii (2×, homozygous recessive, yy) were used as seed parents in experiments combining normal and AD pollen. AD gives a seedling marker to distinguish the pollen parent. In the first experiment, flowers were pollinated daily for one, two, three or four days. AD and normal V. elliottii pollen were applied on separate days. The last day of each treatment received the opposite genotype from the previous day(s). The first pollination gave as many, or more, seedlings as later applications but the number of seeds per fruit was higher with multiple pollen applications. The second experiment used pollen from normal V. corymbosum (4×) alone or mixed with AD pollen from the 2× clones. Results depended on the seed-parent genotype. There was no difference between treatments in the number of hybrids produced by W81-1, which tends to set fruit even with only one seed per berry. Only mixed pollen yielded hybrids from clone FL83-139, which was never observed to produce berries with only one seed; apparently the mentor AD pollen helps to set the fruit, thereby allowing the rare hybrid seeds to develop.

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Timothy F. Wenslaff and Paul M. Lyrene

A study was done to test whether inheritance is tetrasomic or disomic in tetraploid Fl hybrids between 2x Vaccinium elliottii Chapm. and 4x V. corymbosum L. Seventeen Fl hybrids derived from V. elliottii homozygous for recessive anthocyanin deficiency (AD) were confirmed by isozyme analysis and, where V. elliottii was the seed parent, by the presence of anthocyanin. Fertile hybrids with high pollen stainability were assumed to be 4x and duplex for the AD allele, having arisen from 2n gametes in V. elliottii. In nine Fl × Fl crosses, all progeny populations segregated for AD phenotype at or above the expected tetrasomic ratio of 1 AD:35 normal; no AD would be expected with disomic inheritance. Tetraploid AD progeny were used in testcrosses on sixteen Fl hybrids in 1991. Progeny segregated tetrasomically, 1 AD:5 normal. Isozyme loci PGD-2 and PGI-2 also segregated tetrasomically.

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Timothy F. Wenslaff and Paul M. Lyrene

Two diploid yellowleaf Vaccinium elliottii Chapmn. clones were pollinated with pollen from the tetraploid southern highbush cultivar `Misty' (largely V. corymbosum L). These interspecific crosses, which normally yield few hybrids because of a triploid block, were made with and without the use of V. elliottii mentor pollen mixed with V. corymbosum pollen. Mentoring had no effect on the number of hybrids produced when V. elliottii `Silverhill' was the seed parent, but when V. elliottii `Oleno' was the seed parent, no hybrids were produced unless mentor pollen was utilized. The difference was postulated to be a greater ability to produce one-seeded berries in `Silverhill' than in `Oleno'.