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  • Author or Editor: Tilin Fang x
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Bermudagrass (Cynodon spp.) is a drought-resistant warm-season turfgrass adapted to the southern and transitional zones in the United States. Multiple hybrid cultivars have been developed and released for use as turfgrass, and others are still undergoing development. Increasing genetic diversity of commercial cultivars is vital to stress tolerance. A DNA profiling study of 21 experimental selections from the Oklahoma State University turfgrass breeding program and 11 cultivars was conducted using 51 simple sequence repeat primer pairs across the bermudagrass genome. A pairwise genetic relationship analysis of the genotypes using 352 polymorphic bands showed genetic similarity coefficients ranging from 0.59 to 0.89. The average pairwise population differentiation values were 0.012 for the 11 cultivars and 0.169 for the 21 selections. A cluster analysis using the unweighted paired group with the arithmetic average method grouped the entries into six clusters. A correlation analysis identified different levels of pairwise genetic relationships among the entries that largely reflected parental relationship. Directional breeding and selection for cold hardiness or drought resistance created progeny that had distinct genetic diversity in the tested bermudagrasses. It is evident that an increase in genetic diversity of the existing cultivar pool with the release of one or more experimental selections for commercial use will strengthen and improve bermudagrass systems.

Open Access

Turfgrass varietal identification is critical and allows turfgrass professionals to manage the turf based on the cultural requirements of the variety. On the Oklahoma State University (OSU) Baseball Field (OSUBF) in Stillwater, OK, some bermudagrass (Cynodon sp.) plants exhibited desirable traits but their exact identities were unknown due to the installation of multiple varieties over time. Accordingly, the major objective of this study was to identify if the desirable bermudagrass plants were from commercially available known varieties. Recently, the OSU turf bermudagrass breeding program developed and entered three fairway-type clonal bermudagrasses in the 2013 National Turfgrass Evaluation Program (NTEP) bermudagrass trial: OKC 1131, OKC 1163, and OKC 1302. The secondary objective was to create molecular marker profiles for these three experimental lines. Five OSUBF samples were analyzed using simple sequence repeat (SSR) markers, along with 24 clonal, commercially available turf bermudagrass varieties widely used in Oklahoma, the three OSU experimental clones, six randomly selected single plants from ‘Riviera’, and two controls for common bermudagrass (Cynodon dactylon) and african bermudagrass (Cynodon transvaalensis). SSR marker genotyping data indicated that the five OSUBF plants were clones of an identical bermudagrass. The OSUBF bermudagrass had the same fingerprint as ‘Astro-DLM’ bermudagrass for 14 out of 16 SSRs genotyped. Fifteen out of 30 additional SSR markers also showed differences between the OSUBF bermudagrass and ‘Astro-DLM’. The three OSU experimental clones were different from each other and had different fingerprints from the other tested varieties based on SSR profiles, indicating they are new breeding lines. These four distinct lines have potential to be released as new varieties if they demonstrate superior turf quality traits and adaptation over time.

Free access