Bacterial blight, caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad), is the most common foliar disease of caladium, an ornamental aroid grown for its colorful foliage. The disease can reduce the marketability of caladiums produced as potted plants and lower the yield of caladiums grown for tuber production. Three bacterial strains were isolated from symptomatic caladiums and identified as Xad using fatty acid analysis, carbon source use, and the sequence of the 16S-23S spacer, and tested for virulence against three susceptible cultivars. Two strains were virulent to all of the cultivars; however, one strain was differentially pathogenic, virulent against two cultivars, but not to the usually highly susceptible ‘Candidum’. In greenhouse inoculation tests of 17 cultivars and one breeding line, four cultivars were ranked as highly susceptible, nine as moderately susceptible, and five as resistant. Ten of these cultivars were also evaluated with natural infection in the field with good agreement between the results of the greenhouse and field evaluations. Cultivars White Queen, Florida Red Ruffles, Florida Sweetheart, Candidum Jr., and Mrs. Arno Nehrling have been identified as resistant to bacterial blight in greenhouse or field evaluations and can potentially be used in future breeding efforts to produce improved cultivars.
Teresa E. Seijo, Natalia A. Peres, and Zhanao Deng
Fahrettin Goktepe, Zhanao Deng, Brent K. Harbaugh, Teresa Seijo, and Natalia A. Peres
Caladiums, widely used in containers and landscapes as ornamental plants for their bright colorful leaves, are generally forced or grown from tubers. Commercial production of these tubers in central Florida is through dividing “seed” tubers and growing them in fields. Tuber quality is therefore of critical importance to success in container forcing, landscape use, and tuber production. Fusarium tuber rot (Fusarium solani) has been recognized as the most-destructive disease that affects caladium tuber quality. There is anecdotal evidence from growers indicating the existence of resistance in commercial caladium cultivars. To identify and confirm the source of fusarium tuber rot resistance in caladium, F. solani isolates have been collected from rotting tubers grown under different soil conditions and from different locations. The pathogenecity of these isolates has been tested through artificial inoculation of fresh harvested and/or stored tubers, and a number of highly virulent isolates have been identified. These isolates have been used to refine inoculation and disease evaluation techniques. Two techniques, spraying a conidial suspension onto fresh cut surfaces and inserting Fusarium-infested carnation leaf segments into artificial wounds, have proven to yield consistent resistance/susceptibility ratings among cultivars of known difference in resistance to fusarium tuber rot. Appropriate incubation temperatures and humidity seem to be very critical for disease development and evaluation. The two techniques have been used to evaluate 35 cultivars. Several cultivars, including `Candidum', showed a high level of resistance to fusarium tuber rot, and may be good breeding parent for developing new resistant cultivars.
Zhanao Deng, Brent K. Harbaugh, Rick O. Kelly, Teresa Seijo, and Robert J. McGovern
Caladiums (Caladium ×hortulanum) are popular ornamental plants widely grown for their bright colorful leaves. Pythium root rot, caused by Pythium myriotylum, is one of the few soil-borne diseases in caladium that dramatically reduces plant growth, aesthetic value, and tuber yield. Information on the reaction of caladium cultivars to P. myriotylum is not available, but would be valuable for integrated control of this disease and for breeding new resistant cultivars. Three Pythium isolates obtained from decaying roots of plants collected from a field production site and two greenhouses were evaluated for pathogenicity and potential use in experiments to screen commercial caladium cultivars for resistance. All three isolates were found to be highly virulent; they were able to cause obvious root rotting within 3 to 5 days and severe root rotting and leaf losses on susceptible cultivars within 10 days after inoculation. Nineteen major commercial cultivars were evaluated for their resistance to these isolates. Fifteen of the cultivars were susceptible or highly susceptible to Pythium infection. Four widely grown cultivars, `Candidum', `Candidum Jr.', `Frieda Hemple', and `White Christmas', were found to have a moderate level of resistance (partial resistance) to pythium root rot. Pythium infection also caused leaf discoloration, epinasty, wilting, and collapse. Regression analyses revealed a linear relationship between the root rot and leaf loss severity on Pythium-inoculated plants.
Zhanao Deng*, Brent K. Harbaugh, Rick Kelly, Teresa Seijo, and Robert J. McGovern
Caladiums (Caladium × hortulanum) are widely grown for their bright colorful leaves. Pythium root rot, caused primarily by P. myriotylum, is one of the most important diseases in caladiums. This disease can dramatically reduce plant growth, impact plant aesthetical value, and lower tuber yield. Pythium infection in the roots may also lead to subsequent entry of Fusarium into tubers resulting in tuber rot. There has been a strong interest in the tuber production and greenhouse plant production industries to identify cultivars that are resistant or tolerant to Pythium. However, few studies have been conducted since the pathogen was identified, and little information is available regarding the existence of any possible resistance in commercial cultivars. Pythium isolates were made from diseased plants collected from different sites; their pathogenicity was confirmed using tissue culture-derived plants. Procedures were developed for oogonia spore production, inoculation, and disease severity assessment. Nineteen major commercial cultivars were inoculated at two spore densities and then maintained in greenhouses under growing conditions favorable for root rotting. Plant appearance, leaf characteristics and severity of root rotting were evaluated 2-3 times after inoculation. Observations indicated that the isolates were highly virulent. They induced visible root rot within 3-5 days, and caused a complete loss of the root system and plant death for some cultivars within 2-3 weeks after inoculation. Several cultivars, including `Candidum' and `Frieda Hemple' which are widely grown cultivars, had much less root rot, higher plant survival, and seemed to have moderate levels of resistance.
Fahrettin Goktepe, Teresa Seijo, Zhanao Deng, Brent K. Harbaugh, Natalia A. Peres, and Robert J. McGovern
Fusarium tuber rot, incited by Fusarium solani, is the major cause of losses of tuber quality and quantity in caladium (Caladium ×hortulanum) during storage and production. To develop a reliable inoculation method for evaluating cultivar susceptibility to Fusarium tuber rot and identifying sources of resistance, the effect of temperature on the mycelial growth of F. solani in vitro and on tuber rot in vivo was examined. The optimal temperature was then used to study the aggressiveness of F. solani isolates. The effect of temperature (13, 18, 23, 28, and 33 °C) on radial mycelial growth of nine F. solani isolates in vitro was determined, and all responded similarly to temperature variables, with optimal growth predicted to be at 30.5 °C. The relationship of these temperatures to disease development was then determined for the most aggressive F. solani isolate 05-20 and it was found that disease development in inoculated tubers was greatest at low temperatures (13 and 18 °C). Cold damage to tubers was observed at 13 °C; therefore, 18 °C was chosen for all future disease screening. The aggressiveness of nine isolates was tested on two caladium cultivars. Significant differences among isolates were observed for the diameter of rotted tissue in both cultivars, indicating that choice of isolate was important for screening. Isolates 05-20 and 05-257 were highly aggressive on both cultivars. Tubers of 17 commercial caladium cultivars were inoculated with three isolates (04-03, 05-20, and 05-527) and incubated at 18 °C. The interaction between isolates and cultivars was highly significant (P < 0.0001), indicating that cultivars were not equally susceptible to different pathogenic isolates of F. solani. Lesion diameters differed significantly (P < 0.0001) among cultivars/isolates and ranged from 9.5 mm (‘Rosebud’ and ‘White Christmas’ for isolate 04-03) to 23.9 mm (‘Carolyn Whorton’ for isolate 05-20). The cultivars were ranked for susceptibility to tuber rot within each isolate and the normalized total rank for the three isolates was used to place cultivars into four categories: resistant (‘Candidum’, ‘Rosebud’, ‘White Christmas’, ‘Florida Sweetheart’, and ‘Aaron’), moderately resistant (‘White Wing’ and ‘Red Flash’), susceptible (‘Candidum Jr.’, ‘White Queen’, ‘Red Frill’, ‘Florida Cardinal’, ‘Miss Muffet’, and ‘Postman Joyner’), and highly susceptible (‘Fannie Munson’, ‘Gingerland’, ‘Frieda Hemple’, and ‘Carolyn Whorton’). The availability of these sources of host plant resistance, aggressive isolates, and resistance assessment techniques will facilitate the development of new Fusarium-resistant caladium cultivars.
Zhanao Deng, Brent K. Harbaugh, Rick O. Kelly, Teresa Seijo, and Robert J. McGovern
Caladiums (Caladium ×hortulanum) are widely grown as pot or landscape plants for their attractive leaves. Pythium root rot (Pythium myriotylum) is one of the most damaging diseases in caladium, severely reducing plant growth, aesthetic value, and tuber yield. Twenty-three commercial cultivars were inoculated with three aggressive isolates of P. myriotylum and evaluated for their resistance to root rot. Three cultivars, `Apple Blossom', `Blizzard', and `Etta Moore', were found to have a moderate level of resistance (partial resistance) to pythium root rot. The rest of these cultivars were susceptible or highly susceptible to Pythium infection, losing up to 94% of their root tissue to rotting within 10 days after inoculation. Data indicated a linear relationship between root rot severity and leaf loss severity on Pythium-inoculated plants and highlight the importance of controlling pythium root rot in caladium pot plant and tuber production. Comparison of some recent releases with their parents for pythium root rot resistance suggests the potential of developing new resistant caladium cultivars using the identified sources of resistance.