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Tae-Ho Han, Herman J. van Eck, Marjo J. De Jeu and Evert Jacobsen

An F1 population, derived from an intraspecific cross between two Alstroemeria aurea accessions, was used to map quantitative trait loci (QTL) involved in ornamental and morphological characteristics. One QTL for leaf length was mapped on linkage group three of both parents near marker E+ACCT/M+CGCA-I165 explaining 20% and 14.8% phenotypic variation. Two putative QTL were detected on leaf width on A002-3 and A002-6. One QTL and three putative QTL, involved in the leaf length/width ratio were identified accounting for 46.7% of the phenotypic variance in total. Significant interaction was observed between two QTL, S+AC/M+ACT-I162 and S+AC/M+AGA-I465 in a two-way analysis of variance (ANOVA). For the main color of the flower one QTL and putative QTL accounted for up to 60% of phenotypic variance suggesting simple genetic control of flower color. A two-way ANOVA of these QTL suggested an epistatic interaction. A QTL was detected for color of the inner side of outer lateral tepal with 26.5% of the phenotypic variance explained. This QTL was also associated with main color of the flower just below the 95% threshold value. Two QTL were detected with the Kruskal-Wallis test for the tip color of inner lateral tepal near QTL for other flower color traits. Consequently flower color traits were significantly correlated. A QTL and a putative QTL for the flower size was mapped near marker E+ACCG/M+CGCT-I193 and E+ACCG/M+CGCG-197, respectively. One putative QTL was detected for the stripe width of the inner lateral tepal.

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Hae Keun Yun, Kyo Sun Park, Jeong Ho Roh, Yong Bum Kwack, Ji Hae Jun, Seok Tae Jeong, Han Ik Jang, Seung Heui Kim and Yong Uk Shin

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Hae Keun Yun, Kyo Sun Park, Jeong Ho Roh, Yong Bum Kwack, Ji Hae Jun, Seok Tae Jeong, Seung Hui Kim, Han Ik Jang and Yong Uk Shin

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John A. Juvik, Gad G. Yousef, Tae-Ho Han, Yaacov Tadmor, Fermin Azanza, William F. Tracy, Avri Barzur and Torbert R. Rocheford

This study was conducted to identify the chromosomal location and magnitude of effect of quantitative trait loci (QTL) controlling sweet corn (Zea mays L.) stand establishment and investigate the impact of dry kernel characteristics on seedling emergence under field conditions. Genetic and chemical analysis was performed on two F2:3 populations (one homozygous for su1 and segregating for se1, the other homozygous for sh2 endosperm carbohydrate mutations) derived from crosses between parental inbreds that differed in field emergence and kernel chemical composition. A series of restriction fragment-length polymorphism (RFLP) and phenotypic markers distributed throughout the sweet corn genome were used to construct a genetic linkage map for each population. F2:3 families from the two populations were evaluated for seedling emergence and growth rate at four locations. Mature dry kernels of each family were assayed for kernel chemical and physiological parameters. Composite interval analysis revealed significant QTL associations with emergence and kernel chemical and physiological variables. Improved emergence was positively correlated with lower seed leachate conductivity, greater embryo dry weight, and higher kernel starch content. QTL affecting both field emergence and kernel characteristics were detected in both populations. In the su1 se1 population genomic regions significantly influencing emergence across all four environments were found associated with the se1 gene on chromosome 2 and the RFLP loci php200020 on chromosome 7 and umc160 on chromosome 8. In the sh2 population the RFLP loci umc131 on chromosome 2 and bnl9.08 on chromosome 8 were linked to QTL significantly affecting emergence. Since seedling emergence and kernel sugar content have been shown to be negatively correlated, undesirable effects on sweet corn eating quality associated with each emergence QTL is discussed. Segregating QTL linked to RFLP loci in these populations that exert significant effects on the studied traits are candidates for molecular marker-assisted selection to improve sweet corn seed quality.