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T.J. Miesle, A. Proctor, and L.M. Lagrimini

The activity, isoenzymes, and histochemical location of peroxidase were studied in developing highbush blueberries (Vaccinium corymbosum L.). Total peroxidase activity increased during development when expressed on a fresh-weight basis, reaching a maximum in red berries and then declining. When peroxidase activity was expressed per berry it did not decline after the red-berry stage. Most of the enzyme was ionically bound to cell walls throughout development, with the number of isoenzymes increasing with maturity. Histochemical localization of peroxidase showed that enzyme activity spread throughout the berry during development.