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- Author or Editor: T. W. Edwards Jr. x
Abstract
Permanent microscopic slides generally consist of mounting stained tissues in Canada balsam or resin and protecting them with a cover glass. Application of the correct amount of mounting material is necessary to prevent cleaning of excess resin from slides. In addition, air bubbles are often caught under the cover glass and cannot easily be removed. Fast-drying liquid plastics offer a simple technique for solving this problem. Satisfactory permanent slides have been made by using a form of Lucite (methyl methacrylate polymer) to mount tissues (1). This form was soluble in xylene to yield 30 to 40% solutions (w/v).
Abstract
Development from free nuclear to completely cellular endosperm was observed in ‘Flordasun’ and ‘Early Amber’ peaches and ‘Sunred’ nectarine. The free nuclear stage was present at 33, 38 and 49 days before first harvest, respectively, after which cytokinesis began. Approximately a third of the fruit had completely cellular endosperm at 23, 32 and 43 days before first harvest, at which times seed lengths and fruit diameters were 13.5 and 24.1 mm, 13.2 and 22.9 mm, and 11.4 and 20.5 mm, respectively. During cytokinesis, fruit and seed sizes were larger in the varieties with a shorter developmental cycle. Endosperm was completely cellular in all fruit of each variety at 19, 30 and 39 days and early stages of stone hardening was observed at 21, 30 and 43 days before first harvest. Seed length, fruit diameter and fruit volume were highly correlated within each variety and these parameters were related to the stages of endosperm development.
Abstract
Seed development of blueberry (Vaccinivm spp.) from pollination to fruit maturity was investigated for Florida selections ‘4-15’ and ‘4-71’ (2n=4x=48); and Bluegem’ (2n=6x=72). Embryo abortion was observed in each immediately following fertilization, at the end of stage I of fruit development, and during early stage II of fruit development.
Abstract
Repeatability of scoring and inheritance of 5 characters were studied in highbush type blueberry progenies in 2 years. In the repeatability study, fruit size, scar and plant vigor scores were more consistent than fruit firmness and color. Plant vigor scores were high and fruit scar scores were low both years. In the inheritance study, cultivars ranked similarly for each character by either their phenotypic score or general combining ability effects. Mean square variances of general combining ability were larger than specific combining ability for fruit size, firmness, color and plant vigor. Mean square variance of specific combining ability was high for fruit scar. Heritability estimates for blueberry were high for fruit size, moderate for fruit color, low for fruit firmness and scar and lower for plant vigor.
Abstract
Growth cycle studies of blueberry fruit were investigated for 3 Florida selections ‘4-15’, ‘4-71’ and ‘Bluegem’ (‘6-164’) which differed in the length of time from an thesis to maturity. The 3 stages of fruit development were exemplified by a double-sigmoid curve. Fruit size increase in stage I was due primarily to cell division and in stage III to cell enlargement. Delayed growth rate in stage II was accompanied by rapid development of the embryo and endosperm. Duration of individual stages varied with the clone and temperature and the length of each growth stage was important in determining the time required from anthesis to fruit maturity.