High-value crops (tobacco and sweet corn) often receive high levels of N fertilizer during the growing season rather than risk yield and/or quality reductions. Following harvest, small-grain winter cover crops are sown to reduce soil erosion and recover residual fertilizer N. Fall cole crops, such as cabbage, grow rapidly in early fall, respond well to N fertilization, and have the potential to be sold for supplemental income. The objectives of this study were to 1) compare fall cabbage and winter rye as scavengers of residual fertilizer N and 2) determine if a relationship between fall soil mineral-N (NO– 3 +) levels and fall cabbage yield response to N fertilization exists. Soil mineral N levels following sweet corn and tobacco ranged from 22 to 53 mg·kg–1 in the surface 30-cm and declined with depth. Fall cabbage appeared to be as effective as rye at reducing soil mineral N levels. No fall cabbage dry matter yield response to applied N was measured in 1993 and 1995. However, following sweet corn in 1994, a small cabbage yield response to N at 56 kg·ha–1 was measured when the soil mineral level, prior to fall fertilization, was 22 mg·kg–1.
David C. Ditsch and Richard T. Jones
T. Jones, J. Strang, G. Brown and P. Wolfe
Kentucky is one of seven states in the southeast evaluating 13 Asian pear cultivars for suitability to the region. The cultivars were planted on a (20′ × 10′) spacing in 1989 at three separate locations. Data on time of bloom, tree growth, fire blight susceptibility and fruit quality and yield were collected. This study demonstrates the variability seen in Asian pear cultivars in response to site. There was a significant site by cultivar interaction for fire blight. The Princeton site had significantly more fire blight than either Lexington or Quicksand. Four cultivars, Niitaka, Shin Li, Shinko and Shinseiki had low fire blight ratings which were not significantly different between the three sites. Asian pear growth rates were significantly different between the three sites, but cultivar growth rates were not. Tree growth rate showed a significant negative correlation to fire blight rating. That is infected trees did not grow much. Initial findings show Shinko, Shinseiki and Niitaka to have some tolerance to fire blight spread and to produce good yields of attractive fruit. However, Niitaka had a very tough skin with a tendency towards fruit cracking. The cultivar Shin Li which also had fire blight tolerance did not produce fruit or flowers.
Richard E. Bir, T. G. Ranney and R. K. Jones
Twelve shrub rose cultivars were evaluated for pest resistance in the southern Blue Ridge mountains under high humidity and rainfall (1.34 inches per week average during the 1994 and 1995 growing seasons). `Albo plena', `Blanc Double de Coubert', `Fru Dagmar Hastrup', `Roseraie de l'Hay', R. rugosa `Alba', `Sarah van Fleet', and `Topaz Jewel' were highly resistant to black spot and Cercospora sp. leaf spot. `Alba Meidiland', `Linda Campbell', `Pink Meidiland', and `Scarlet Meidiland' were susceptible, while `Bonica' displayed intermediate resistance to both diseases. `Sarah van Fleet' foliage and the flowers of `Albo plena', `Blanc Double de Coubert', and R. rugosa `Alba' were damaged by Japanese beetle feeding. No other cultivars were damaged by Japanese beetles.
Sanford D. Eigenbrode, John T. Trumble and Richard A. Jones
Accessions of Lycopersicon esculentum var. cerasiforme (Dun.) A. Gray (cer) and L. pimpinellifolium (Jusl.) Mill. (pimp), sustained significantly less damage to fruit by beet armyworm [Spodoptera exigua (Hiibner)] than standard cultivars and breeding lines of L. esculentum Mill. (esc) under natural infestations in 1990 and 1991 in southern California. The dwarf vine cherry cultivar Tiny Tim also sustained less damage than the standards. Accessions of esc with various monogenic mutations sustained at least as much beet armyworm damage as did standard cultivars. The percentage of fruit damaged was significantly correlated with vine weight, weight per fruit, number of fruit, and the fruit-foliage weight ratio (Pearson's coefficients, respectively: -0.533, 0.450, -0.483, 0.390, n = 37). In laboratory assays, survival of beet armyworm was significantly lower (5% of susceptible& growth rates were significantly lower, and development time was significantly longer on the fruit of resistant `Tiny Tim' and LA 1320 cer than the fruit of 11 other test lines. There were no substantial differences in beet armyworm survival on the foliage of the test lines. In the field trials, there were also significant differences among the test lines in damage by Liriomyza species and hemipteran pests. Lines with genes for increased densities of nonglandular leaf trichomes (especially LA 1663) were generally less damaged by Liriomyza than other lines. Damage by hemipterans was correlated with vine and fruit size, fruit count, and fruit-foliage weight ratio in 1991, but high intraseason variability prevented clear identification of test lines resistant to these pests.
J. Strang, J. Hartman, R. Bessin, T. Jones, G. Brown, T. Barnes, T. Yankey and J. Snyder
Four different netting types were evaluated in the field for excluding Japanese beetles and green June beetles from `Dirksen' thornless blackberry plants. These nets were bird net, crop net, rack mesh, and Agryl P17. Observations were made in an unreplicated trial on `Reliance' grapes using OV3018 and OV7100 nets in addition to those listed. Plants were not sprayed with insecticides or fungicides after net application. Rack mesh appears to be the best net of those evaluated during a dry season for excluding Japanese beetles and green June beetles on thornless blackberries and grapes. Plants covered with rack mesh had minimal fruit and foliage damage due to insects and fruit rot. The use of rack mesh eliminated the need for insecticide sprays for 53 days on thornless blackberries and 41 days on grapes. Light intensity was reduced by the netting, but did not reduce (hornless blackberry yield or soluble solids; however it did unacceptably reduce `Reliance' grape fruit coloration.
G.R. Brown, J. Hartman, R. Bessin, T. Jones and J. Strang
Apple growers would like to use pesticides efficiently and diminish concerns about food safety and pesticide usage. The 1992 Apple IPM Program objectives were: 1) to demonstrate the application of Integrated Pest Management practices in commercial orchards and, 2) to provide the training and support needed to help these growers become self sufficient in IPM practices. Grower training meetings and regular scouting of the orchards were the primary educational methods. End-of-the-season evaluations of past and disease incidence were made. Except for Frogeye Leaf Spot, there were no significant differences in insect pest, disease levels or in fruit quality attributes in IPM versus standard blocks. The IPM blocks had significantly more mite incidence. Growers did produce commercially acceptable crops using IPM based decisions while reducing the average past control cost by $56 par acre. Educational programs did help growers to be more proficient in making IPM based decisions.
Jon T. Lindstrom, Chih-Hsien Lei, Michelle L. Jones and William R. Woodson
Mature pollen from Petunia hybrida contains significant levels of 1-aminocyclopropane-1-carboxylic acid (ACC), and this ACC is thought to play a role in pollination-induced ethylene by the pistil. We investigated the developmental accumulation of ACC in anthers and pollen. The level of ACC in anthers was very low until the day before anthesis, at which time it increased 100-fold. A 1.1-kb partial ACC synthase cDNA clone (pPHACS2) was amplified from total RNA isolated from mature anthers by reverse transcriptase, followed by polymerase chain reaction using oligonucleotide primers synthesized to conserved amino acid sequences in ACC synthases. The expression of pPHACS2 mRNA during anther development was correlated with the accumulation of ACC and was localized to the pollen grain. The pPHACS2 cDNA was used to identify the PH-ACS2 gene from a library of genomic DNA fragments from Petunia hybrida. PH-ACS2 encoded an ACC synthase transcript of four exons interrupted by three introns. The ACC synthase protein encoded by the PH-ACS2 gene shared >80% homology with ACC synthases from tomato (LE-ACS3) and potato (ST-ACS1a). A chimeric PH-ACS2 promoter-β-glucuronidase (GUS) gene was used to transform petunia and transgenic plants were analyzed for GUS activity. GUS staining was localized to mature pollen grains and was not detected in other tissues. Despite similarities to LE-ACS3, we did not detect GUS activity under conditions of anaerobic stress or in response to auxin. A series of 5-prime-flanking DNA deletions revealed that sequences within the PH-ACS2 promoter were responsible for pollen-specific expression.
D.W. Lickfeldt, N.E. Hofmann, J.D. Jones, A.M. Hamblin and T.B. Voigt
An efficient deoxyribonucleic acid (DNA) extraction procedure that yields large quantities of DNA would provide adequate DNA for a large number of different analytical procedures. This study was conducted to compare three DNA extraction procedures for cost, time efficiency, and DNA content while extracting DNA from Kentucky bluegrass (Poa pratensis L.). Three students at the Univ. of Illinois with varying levels of DNA extraction experience conducted DNA extractions using Plant DNeasy™ Mini Kits, Plant DNAzol® Reagent, and a PEX/CTAB buffer. Costs varied significantly with cost (US$) per DNA sample of $3.04 for the DNeasy™ method, $0.99 for the DNAzol® method, and $0.39 for the PEX/CTAB extraction. The DNAzol® method was the fastest; although extracting 2.8 ng less DNA than the DNeasy™ method, it did not require the use of hazardous organic solvents, and random amplified polymorphic DNA (RAPD) markers were satisfactory for DNA fingerprinting of Kentucky bluegrass cultivars. The PEX/CTAB method, which did not include a tissue homogenization step, did not have reproducible banding patterns due to miniscule and inconsistent quantities of DNA extracted, or possibly due to inadequate purification. The investigator with the least DNA extraction experience was the slowest, while extracting 75% more DNA. All three methods are easily adapted to laboratories having personnel with different levels of experience. The DNAzol® Reagent method should save time and money, with reproducible results when many individual plant samples need to be identified. Chemical names used: potassium ethyl xanthogenate (PEX); cetyltrimethyl ammonium bromide (CTAB)
Suat Irmak, D.Z. Haman, A. Irmak, J.W. Jones, B. Tonkinson, D. Burch, T.H. Yeager and C. Larsen
This research study evaluates the effectiveness of a recently introduced irrigation-plant production system, multipot box system (MPBS), for moderating root zone temperature (RZT) compared with the conventional nursery containers. The study also deals with the development, calibration, and validation of a series of models that can be used to predict maximum (max) and minimum (min) RZTs using commonly available input variables. The Viburnum odoratissimum (Ker.-gawl.) was used as the test plant. Models were calibrated in the fall growing season and validated during the summer. The RZT was used as the dependent variable while the max and min air temperatures (Tmax and Tmin) and/or incoming solar radiation (Rs) were used as independent variables. The color of the MPBS had an effect on plant growth. Plants grown in the white MPBS had higher growth indices, shoot and root dry weights, and number of stems as compared with the plants in the black MPBS or the conventional (control) system (CS). White MPBS maintained cooler RZTs than the max air temperature during both seasons. Also, white MPBS maintained cooler RZTs than the black MPBS and CS during the two seasons. In both seasons, water temperature in the black MPBS was higher than the temperature in the white MPBS contributing to the high RZTs in the black MPBS. The RZT of the black MPBS and CS exceeded the critical value (40 °C), which is cited in the literatures as negatively impacting root growth, water and nutrient uptake, leaf area, plant survival, root and shoot dry weights, water status, and photosynthesis. The RZT in the CS was above 45 °C for most of the summer season and plants were exposed to this extreme temperature for a few hours a day during most of the summer. The white MPBS provided a better environment and enhanced plant growth. For regions where ambient air temperature ranged from 2 to 41 °C, the white MPBS can provide adequate and effective RZT protection for plants grown in No. 1, 3.8-L standard black conventional containers. Predicted RZT values were well correlated with measured values in all systems. Rs did not have an effect on predicting RZTmax in the MPBS treatments. Wind speed did not contribute to predicting RZT in any production systems. The root mean square error between measured and predicted RZT was relatively low ranging from 0.9 to 2.8 °C. Models were able to explain at least 74% of the variability in RZTs using only Tmax, Tmin, and/or Rs. Models developed in this study should be applicable for estimating RZTs when similar management and cultural practices are present. Models of this study are practical, simple, and applicable to predict RZTs where ambient air temperature ranges from 1.9 to 40 °C. Model results should not be extrapolated beyond these limits.
Patrick A. Jones, James T. Brosnan, Gregory K. Breeden, José J. Vargas, Brandon J. Horvath and John C. Sorochan
Divoting is a common occurrence on golf courses and athletic fields. Research was conducted at the University of Tennessee Center for Athletic Field Safety (Knoxville, TN) during 2012–13 evaluating the effects of preemergence (PRE) herbicide applications on hybrid bermudagrass [C. dactylon (L.) Pers. × C. transvaalensis Burtt-Davy, cv. Tifway] divot resistance and recovery. Plots were subjected to the factorial combination of seven herbicide treatments (indaziflam at 35 and 52.5 g·ha−1; prodiamine at 840 g·ha−1; pendimethalin at 3360 g·ha−1; dithiopyr at 560 g·ha−1; oxadiazon at 3360 g·ha−1; non-treated control) and three divot timings [1, 2, and 3 months after herbicide treatment (MAT)]. Rates were based on label recommendations for preemergence crabgrass (Digitaria spp.) control. Herbicides were applied on 15 Mar. 2012 and 2013. Divots were generated using a weighted pendulum apparatus designed to impart 531 J of impact energy to the turf sward with a golf club. Divot resistance was quantified by measuring divot volume at each timing while divot recovery was quantified by measuring turf cover in the divot scar using digital image analysis. All herbicide-treated plots produced divots with volumes ≤ the non-treated control. In 2013, volumes were greater for divots produced 1 MAT (215 cm3) than those created 2 MAT (191 cm3) or 3 MAT (157 cm3). No differences in divot recovery were detected as a result of herbicide treatment in either year. Under the conditions of this study, applications of PRE herbicides at labeled rates did not affect divot resistance or recovery.
Chemical names: N-[(1R,2S)-2,3-dihydro-2,6-dimethyl-1H-inden-1-yl]-6-(1-fluoroethyl)-1,3,5-triazine-2,4-diamine (indaziflam), 2,4 dinitro-N3,N3-dipropyl-6-(trifluoromethyl)-1,3-benzenediamine (prodiamine), N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine (pendimethalin), S,S-dimethyl 2-(difluoromethyl)-4-(2-methylpropyl)-6-(trifluoromethyl)-3,5-pyridinedicarbothioate (dithiopyr), 3-[2,4-dichloro-5-(1-methylethoxy)phenyl]-5-(1,1-dimethylethyl)-1,3,4-oxadiazol-2-(3H)-one (oxadiazon)