Cucumber (Cucumis sativus L.) seedlings were brushed with a suspended bar for 1.5 min twice daily for 12 days (ST) prior to planting. One group of plants was brushed for an additional 10 days (LT) after planting. ST reduced stem length 12 to 28% and shoot dry weight 6 to 24% with `Kurume-ochiai-H' least responsive. ST reduced the numbers of female flowers on lateral shoots of `Ritsurin' and `Chikanari-suhyoh' while LT affected `Nanshin' and `Chikanari-suhyoh'. Brushing did not affect the total number of fruits or mean fruit size or weight of any cultivar, but both ST and LT decreased the total yield of `Ritsurin' grown in a plastic house. Brushing provides good growth control of containerized cucumber transplants with some responses differing among the cultivars. [Project funded by JSPS and Monbusho.]
J.G. Latimer, T. Johjima and K. Harada
Y. Wakasa, R. Ishikawa, M. Niizeki, T. Harada, S. Jin, M. Senda and S. Akada
Sequencing of the sequence tagged site-DNA marker fragments linked to the fruit skin-color gene in apple revealed two fragments containing insertions that are associated with yellow skin color, and are alleles of the red skin gene (Rf). One fragment resulted from the insertion of a 76-bp inverted repeat, whereas the 163 bp in the other fragment was characterized as a mobile element because of the presence of target site duplication. A database search found this latter element in the 5' flanking regions or intron of six apple genes. DNA blot analysis revealed that the element is highly reiterated, with a copy number of between 5000-6000 in the genome of Maloideae (pome) fruit trees. We named this element Majin. Inter-transposon amplified polymorphism using primers from this element resulted in polymorphic bands among apple cultivars.