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Abstract
The earliest description of ethylene-induced flower fading is in a monograph by George Fahnestock, written in 1858, after his collection of rare, exotic plants was exposed accidentally to illuminating gas leaking from a broken street main (15). Ethylene was later identified as the biologically active component of illuminating gas by Neljubow, in 1901 (23), during his study of a strange growth habit of etiolated pea seedlings raised in laboratory air. A winter disease of carnations, called “sleepiness,” was traced to ethylene, present in railcars or homes as a byproduct of incompletely burned or leaking illuminating gas (10, 16).
U.S. regulations prevent importation of fresh horticultural commodities that have not received an approved quarantine treatment assuring 100% mortality of potentially invasive insect pests. Because imported mangoes are likely to be infested by the Caribbean fruit fly (Anastrepha suspensa Loew) and other tropical fruit flies such as A. ludens Loew, A. striata, A. distincta, A. fraterculus, A. serpentina, or A. oblique, they must be hot-water treated prior to shipment in order to satisfy quarantine requirement. Hot water treatment often damages the fruit, especially if it is not fully mature. Hypobaric (low pressure = LP) intermodal shipping containers developed by the VacuFreshSM Corp. preserve fresh commodities, such as horticulturally mature mangoes, far longer than is possible using other technologies. We tested the ability of over 70,000 Caribbean fruit fly eggs and larvae to survive a simulated optimal hypobaric condition for shipment of mangoes (15 mm Hg, 98% RH, at the lowest, safe non-chilling temperature, 13 °C). A. suspensa eggs or larvae were maintained on agar media, flushed with one air change per hour at the storage pressure, and shielded with Mylar to prevent radiant heat uptake and limit evaporative cooling. Nearly 98% of the eggs and larvae were killed within 1 week at 15 mm Hg in eight replicated experiments. All eggs were killed at that pressure by 11 days, whereas a significant number survived at ambient pressure. Shipment of fresh produce using this technology promises to provide quarantine control while preserving the freshness of fully mature tropical fruits and vegetables.
U.S. regulations prevent importation of fresh horticultural commodities that have not received an approved quarantine treatment assuring 99.999% (Probit 9) mortality of potentially invasive insect pests. Because imported mangoes (Mangifera indica) are likely to be infested by the caribbean fruit fly (Anastrepha suspensa) and other tropical fruit flies in the Americas, such as the mexican fruit fly (A. ludens), guava fruit fly (A. striata), inga fruit fly (A. distincta), south american fruit fly (A. fraterculus), sapote fruit fly (A. serpentina), and the west indian fruit fly (A. obliqua), they must be hot-water treated prior to shipment in order to satisfy quarantine requirement. Hot water treatment often damages the fruit, especially if it is not fully mature. Hypobaric [low pressure (LP)] intermodal shipping containers developed by the VacuFresh Corp. preserve fresh commodities, such as horticulturally mature mangoes, far longer than is possible using other technologies. We tested the ability of caribbean fruit fly eggs and larvae to survive simulated optimal hypobaric conditions for shipment of mangoes [15 and 20 mm mercury (Hg), ≥98% relative humidity, 13 °C (the lowest, safe nonchilling temperature)]. Caribbean fruit fly eggs or larvae were maintained on agar media, flushed with one air change per hour at the storage pressure, and shielded with Mylar to prevent radiant heat uptake and limit evaporative cooling. Nearly 98% of the eggs and larvae were killed within 1 week at 15 and 20 mm Hg in nine replicated experiments. All eggs were killed by 11 days with a predicted kill of 99.999% of the eggs by 9.4 days in 15 mm Hg and 10.6 days in 20 mm Hg LP (based on Probit 9 statistical analysis), whereas a substantial number of eggs survived to 14 days at atmospheric pressure (760 mm Hg). Shipment of fresh produce using this technology promises to provide quarantine control while preserving the freshness of fully mature tropical fruits and vegetables.
Abstract
The main evidence for a single mechanism of ethylene action is the observation that nearly all responses to the gas have the same dose response curve (8), suggesting a single type of receptor molecule. If so, the case is similar to that of phytochrome where one biochemical change produces a multitude of secondary changes resulting in a variety of physiological responses depending upon the tissue involved. We have chosen to use the etiolated pea seedling to investigate the primary and secondary actions of the gas because all parts of this plant have been extensively studied and are highly responsive to ethylene. When this seedling is exposed to ethylene stem growth slows, the hook tightens, the subapex swells and nutates horizontally, root growth slows and the zone of elongation swells, root hairs form, lateral root formation is inhibited, and the root tip bends plageotropically. The causes of these changes are to be found in the effects of ethylene on cell division, cell expansion, and auxin transport.