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  • Author or Editor: Sorua L. Maki x
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Endogenous gibberellins of chrysanthemum [Dendranthema ×grandiflorum (Ramat)] cv. Bright Golden Anne were characterized in apices from plants grown under control and CuSO4 spectral filters. Expanding shoots were separated into young expanding leaves and apices. Methanolic extracts of young expanding leaves were purified by solvent partitioning, PVPP column chromatography and reversed-phase high performance liquid chromatography. Two bioactive regions corresponding to the HPLC retention times of GA1 and GA19 standards were detected in fractions using the recently-developed non-dwarf rice bioassay. Di-deuterated internal standards of GA12, GA53, GA19, GA20, and GA1 were added to similar extracts of shoot apices. The presence of endogenous GA53, GA19, GA20, and GA1 in chrysanthemum apices was confirmed by isotope dilution using gas chromatography-mass spectrometry-selected ion monitoring and Kovats retention indices. In a preliminary quantification study, GA20 and GA1 levels were found to be higher in apices from plants grown under control filters while GA19 levels were higher in apices grown under CuSO4 filters. The possibility that light transmitted through CuSO4 filters alters gibberellin levels in shoot apices is discussed.

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