The pinwheel flowering African violet `Silver Summit', a periclinal chimera, has bicolor flowers with violet-blue 93B corolla segment margins and white 155B central stripes. Several off-types were produced during in vitro culture of `Silver Summit', the two of greatest potential value having reversed color patterns with violet-blue stripes and white margins. The off-types varied in color, one with deep violet-blue stripes (DR, dark reverse) and the other with lighter stripes of the same color (LR, light reverse). Unexpanded inflorescences of both off types were cultured on Murashige and Skoog medium containing 0.1 mg/L benzyladenine and 0.1 mg/L naphthaleneacetic acid. Plantlets resulting from expansion and multiplication of the dormant buds in the inflorescences were removed, acclimated, and grown on to flowering. Thirteen LR inflorescences produced 55 plants; 51 were true to type and four had solid violet-blue flowers (non-chimeral). Thirteen DR inflorescences produced 64 plants; only eight were true to type, 17 produced solid violet-blue flowers, 38 produced flowers with mixtures of the DR chimeral pattern and solid violet-blue flowers, and one was solid white flowering. To visualize the chimeral arrangement of the meristems of the off-types, flower patterns of all plants were recorded and “fl oral maps” were constructed. Floral maps of LR were constant from plant to plant and varied little as the plants aged, indicating LR to be a stable periclinal chimera. Floral maps of DR were highly variable from plant to plant, and changed considerably over time indicating that the DR meristems were less stable.
The pinwheel-flowering African violet `Silver Summit', apericlinal chimera, has bicolor flowers with violet-blue corolla segment margins and white central stripes. Several off types were produced during in vitro culture of `Silver Summit'—solid violet-blue flowering from leaf or petiole explants, solid white flowering from petiole core explants, and two reverse pinwheel flowering types. The reverse pinwheel types varied in color; one had deep violet-blue stripes (DR, dark reverse) and the other had lighter stripes of the same color (LR, light reverse). Plantlets derived from inflorescence culture (Murashige and Skoog medium containing 0.1 mg/1 NAA, 0.1 mg/1 BA) were grown on to flowering. Of 55 plants from LR inflorescences, 51 were true-to-type. The remainder were solid violet-blue flowering. Of 64 plants from DR inflorescences, only 8 were true-to-type, 17 were solid violet-blue flowering, one was white flowering, and 38 were mixed flowering. In vitro inflorescence culture can be used to clone pinwheel flowering African violets; however, chimeral stability of the plant produced varies between clones.