The objective of this study was to determine best production practices for five different specialty cut flower species at an altitude of 7200 ft. Region-specific information about cut flower production is important because of unique environmental conditions. We grew five specialty cut flower species in two different growing environments: a greenhouse and a high tunnel. Flowers were grown year-round in the greenhouse and during late spring through fall in the high tunnels. We also used pinching as another production method for the potential increase in branching. The goals were to test the effects of species, growing environment, and pinching on the days from sowing to harvest, stem length, number of stems cut per plant, and marketable yield. Experiments were conducted at the University of Wyoming Laramie Research and Extension Center in Laramie, WY, to assess the potential for producing specialty cut flowers for local consumption. The species used in this study included ‘Princess Golden’ pot marigold (Calendula officinalis), ‘Lucinda Mix’ stock (Matthiola incana), ‘Double Mix’ strawflower (Helichrysum bracteatum), ‘Dara’ ornamental carrot (Daucus carota), and ‘Celway Mix’ cockscomb (Celosia argentea). Results showed significant species × environment and season interactions, indicating the importance of species and production practice selections. We successfully sold the cut flowers to the university student farm for community-supported agriculture shares and farm market sales, as well as to a local florist for use in floral arrangements. This study concluded that careful species selection for season and growing environment is essential for the successful integration of these niche cut flowers into current or future greenhouse and high-tunnel production in Wyoming.