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Tomatoes (Lycopersicon esculentum Mill. cv. Heinz 1350) were grown under several different N regimes, and used as stock plants for vegetative propagation. The dry wt of vegetatively-propagated shoots was closely related to the crude protein content of the stock plants. The dry wt of roots from terminal cuttings was positively correlated with the crude protein content of the stock plants. The crude protein accumulated by the new shoot was proportional to the size of the shoot.

Open Access

The genus Asimina has the only temperate representatives of the tropical Annonaceae, or Custard Apple family, and includes eight species that are indigenous to North America. The North American pawpaw Asimina triloba (L.) Dunal has the largest edible fruit native to the United States and is the best-known of these species. The USDA National Clonal Germplasm Repository for Asimina species is located at Kentucky State Univ. (KSU); therefore, assessment of genetic diversity is an important research priority for KSU. The inter-simple sequence repeat PCR (ISSR-PCR) methodology has been used successfully to characterize genetic diversity within and among populations of many plant species. The objective of this study was to assess the utility of ISSR markers in evaluating genetic relationships in members of the Asimina genus, as well as closely related tropical relatives in the Annona genus. Leaf samples were collected from three plants each of Asimina longifolia, A. obovata, A. parviflora, A. reticulata, A. tetramera and A. triloba. Leaf samples were also collected from three plants each of Annona cherimola, A. squamosa, A. reticulata, A. muricata, A. glabra, A. diversifolia, and A. montana. DNA was extracted from leaf samples and subjected to ISSR-PCR using the REDExtract-N-Amp™ Plant PCR Kit. DNA samples were screened with ISSR primers using the Univ. of British Columbia microsatellite primer set #9. Three primers, UBC812, UBC841, and UBC873 were found to produce 84 scorable ISSR markers and allowed the determination of genetic relationships among Asimina and Annona members examined.

Free access

Container production has many advantages over traditional in-ground (field) production, including less damage occurring to the root system when transplanted, better establishment after transplanting, decreased labor and land acquisition costs for production, and increased product availability and longevity in the retail market. Growing plants in containers, however, alters root growth and function and can change root morphology. Numerous factors influence root growth in containers. Roots of container-grown plants are subjected to temperature and moisture extremes not normally found in field production. The effects of substrate aeration (Ea) as well as water holding capacity (Pv) interact with different pot characteristics, resulting in changes to root morphology. Successful plant establishment after transplanting is often linked to root health. This review focuses on the roles of substrate physical and chemical properties, container characteristics, and temperature in altering root growth in container-grown woody nursery crops. Root circling, planting too deeply or “too-deep syndrome” (TDS), and the use of composts as container substrates will also be examined.

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