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Abstract
Differences in germination response at 35°C were found in tomato (Lycopersicon esculentum Mill.). Varieties with tolerance to germination at high temperature were characteristically cold-germinating types with the notable exception of the variety, Fireball. The ability to respond at high temperature was not found to be associated with earliness.
Abstract
Selected F3 and F4 lines of different origin were evaluated at high temperature (26° night and 35°C day) in the greenhouse along with several known heat-sensitive and heat-tolerant tomato (Lycopersicon esculentum Mill.) cultivars. A number of the selected lines had fruit set comparable to the tolerant cultivars Saladette, Ohio 7663, BL 6807, and Chico III. The expected greater fruit set of the tolerant cultivars vs. that of the sensitive cultivars was observed. High fruit set in this study was not associated with high seed number in the majority of tolerant cultivars evaluated.
Abstract
‘Ohio 7681’ is an early-main season tomato (Lycopersicon esculentum Mill.) suitable for hand or machine harvest, released by the Ohio Agricultural Research and Development Center, September 1980. Canning quality favors juice and puree production.
Abstract
‘Ohio 7870’ is an early-main season tomato (Lycopersicon esculentum Mill.) suitable for machine harvest with fruit qualities for the cannery that make it especially suitable for the production of juice, sauce, and coreless whole-canned tomato (whole-pack). It was released by the Ohio Agricultural Research and Development Center, June 1981.
Abstract
‘Ohio 7663’ is an early-main season, machine-harvest tomato (Lycopersicon esculentum Mill.) with canning quality suitable for coreless whole-pack product. It was evaluated with good results, in the Northern Tomato Exchange Program (N.T.E.P.) trials in 1977 and 1978 and in other tests in Midwestern United States and Canada.
Abstract
‘Ohio 736’ is an early, well-adapted machine-harvest tomato (Lycopersicon esculentum Mill) with superior canning quality.
Unilateral incompatibility has limited the direction of crossing between L. esculentum and L. hirsutum; the latter can only serve as the pollen parent. In an attempt to introduce the L. hirsutum cytoplasm into L. esculentum, thirty-three somatic hybrid plants have been regenerated following four separate fusions between leaf protoplasts of L. hirsutum PI 126445 and etiolated hypocotyl protoplasts of L. esculentum (`OH7870', `OH832', and `OH8245'). A 33% PEG solution supplemented with 10% DMSO was used as the fusogen. Selection of fusion products was based on treatment of L. hirsutum protoplasts with 1 mM iodoacetic acid and non-regenerability of the L. esculentum genotypes. Hybridity was initially confirmed by intermediate morphology, including leaf shape, type of trichomes, flower shape, stigma placement, and fruit size and color. Isozyme analysis for GOT, PGM, and 6-PDH verified hybridity. Six of the hybrids produced viable seed upon selfing. At least some of the hybrids contained chloroplast DNA from L. hirsutum, indicating that the wild species cytoplasm may be present in these plants.
L. hirsutum has been previously reported as recalcitrant to culture and plant regeneration. We have modified tomato protoplasm culture protocols and obtained high frequencies of plant regeneration from leaf protoplasts of L. hirsutum PI 126445, LA 94, and LA 1393, as well as from 8 interspecific hybrids of PI 126445 (male parent) with L. esculentum `Floradade', `Marglobe', `Tropic', `OH7870', `OH7983', `OH832', `OH8243', and `OH8245'. Protoplasts were isolated from 3-week old low light pretreated seedlings and cultured in modified LCM containing 1 mg/L NAA 0.5 m /L BA, and 0.5 mg/L 2,4-D. Cultures were kept in the dark at 30 C, diluted every 3 days with LCM containing only 0.75 mg/L BA and gradually moved to the light. After 2-3 weeks, colonies of 1-2 mm were transferred to solid MS medium containing 0.5 mg/L BA and 0.05 mg/L NAA. Calli containing dark green bud primordia were then placed on MS with 2% sucrose and 2 mg/L zeatin riboside for shoot production.