Search Results
Abstract
Effects of NAA and BA in media on plantlet vigor and suitable methods of acclimatization to field conditions for garlic (Allium sativum L. ‘Katei’), obtained from shoot apices in vitro, were studied. After 60 days of culture, plantlet growth was normal and vigorous when produced on Murashige and Skoog (MS) medium containing both NAA and BA at 0.01 mg·liter-1. Bulb formation was observed in plantlets after 60 days by transferring to the medium containing both NAA and BA at 0.01 mg·liter-1 or NAA at 0.1 mg·liter-1 and BA at 0.01 mg·liter-1. For acclimatization, aseptic bulb-lets were transferred to subculture medium containing both NAA and BA at 0.1 mg liter-1; plants were obtained after 50 days of acclimatization on rockwool, vermiculite, or soil. All of the plants survived under acclimatization conditions of 20C with a 16-hr day-length of 20 μmol·s-1m-2. Rockwool was found to be the best medium for acclimatization, followed by soil and vermiculite. Chemical names used: 2-(l-naphthyl)acetic acid (NAA); N-phenylmethyl)-1H-purin-6-amine (BA).