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More than 170 pecan [Carya illinoensis (Wangenh.) K. Koch] cultivars were evaluated formalate dehydrogenase, phosphoglucose isomerase, phosphoglucomutase, leucine aminopeptidase (LAP), and diaphorase (DIA). Isozymes of LAP were observed in two regions after starch gel electrophoresis. The faster region of activity (Lap-1) was polymorphic and consistently expressed in leaves, wood, and roots. Controlled crosses suggest that Lap-1 is simply inherited and controlled by at least two alleles. DIA was well resolved and storable only from leaf material and produced a complex banding pattern. The ability to differentiate among cultivars by isozymes was good. The 177 cultivars sorted into 72 classes. Forty of the cultivars (23%) possessed a unique series of isozyme patterns. Most cultivars (124 of 177) shared common banding patterns with less than four other cultivars. From the inheritance models of four isozymes, some historical pedigrees can be questioned. Most notably,' Western Schley' could not have been parented by `San Saba' based on the inheritance of Mdh-1 and Lap-1.