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  • Author or Editor: Russell Nagata x
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Damage due to leaf mining by Liriomyza trifolii (Burgess) is a major problem in many leafy vegetables, especially lettuce. A hierarchy of feeding preference of leafminer on lettuce, Lactuca sativa L. cultivars 'Valmaine', `Parris Island Cos', `Floricos 83', and `Tall Guzmaine' was determined. Leafminers were given a choice of two plants. Observations on the number of times that leafminers probed the leaf surface to feed or oviposit in each plant was counted. `Tall Guzmaine' was significantly preferred in all combinations. Probe ratio of `Tall Guzmaine' verses the other cos lines ranged from 4:1 to 90:1. There were no significant difference between the other three lines, although `Valmaine' had the lowest count in most cases. Based on the pedigrees of the cultivars tested, the observed preferences appears to be under genetic control.

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Host plant resistance is a key element in a viable integrated pest management plan. Resistance to plant feeding was observed on Valmaine cos lettuce, Lactuca sativa L. to the banded cucumber beetle (BCB), Diabotica balteata (LeConte). In no-choice feeding evaluations, adult BCB contained on three week old Valmaine plants gained less weight, died and fed less than individuals contained on susceptible Tall Guzmaine cos lettuce. Individual female BCB held on Valmaine plants also did not have egg development as in those individual held on Tall Guzmaine. Based on weight gain and feeding damage F1, F2, and F3 segregation data indicates that the resistance factor is recessive in inheritance and controlled by more that one gene.

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The objectives of this research were to rank the relative shade tolerance of some new st. augustinegrass (Stenotaphrum secundatum) cultivars and to determine what levels of shade the various cultivars can tolerate. Two consecutive studies were conducted in a glasshouse at the University of Florida Turfgrass Research Envirotron. Cultivars tested were `Bitter Blue', `Floratam', `Palmetto', `Seville', and `1997-6'. Grasses were grown in full sun or under shade structures that provided 30%, 50%, or 70% shade. In trial 1, `Seville' and `1997-6' generally provided best performance under increasing shade, with worst responses seen in `Floratam'. `Seville' and `1997-6' were predicted to maintain an acceptable quality rating of 6 at all shade levels. In trial 2, `Floratam' again had lowest visual quality scores. At 30% shade, `Seville', `Palmetto', and `Bitter Blue' ranked in the highest category, while only `Seville' and `Bitter Blue' had highest rankings at 50% shade. Reduced density was a major factor in turf decline as shade increased. Most of the cultivars performed best under some degree of shade. With the exception of `Floratam', acceptable visual scores were maintained at shade levels exceeding 60% in trial 1 and up to 61% in trial 2.

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Recently, an increasing number of restaurants in Palm Beach County, Florida, have been requesting squash (Cucurbita pepo) flowers from local vegetable growers. Typically, current field-grown squash cultivars produce a higher ratio of female to male flowers, with the emphasis on fruit production. However, a market for squash blossoms indicates a need for cultivars that produce higher numbers of consistently developing male flowers throughout the growing season. In order to evaluate male squash blossom production, 10 squash cultivars, including yellow-summer, zucchini, round, and scallop-types, and one compact-type pumpkin, were field-grown during the 2005–06 growing season. The average number of male flowers per plant by week was recorded for 7 weeks, starting when the first male flowers were identified within the entire trial. In addition to blossom counts, flower traits, such as bell height, depth, volume, and weight were also recorded. Preliminary results from the 2005 season indicate that the commercial yellow-summer squash cultivars, Mulitpik and Early Prolific Straightneck, and the zucchini cultivars, Jaguar and Raven, produced fewer male flowers on a week-by-week and total basis. The cultivar, White Bush Scallop, produced significantly more male flowers then any other entry, with an average of 9.8 male flowers per plant per week. Little or no difference was seen in bell height and depth among the 11 cultivars; however, two cultivars, Costa Romanesque and Hybrid Pam (compact pumpkin type) had significantly greater bell volumes and weights, indicating a much larger blossom size.

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Temperature is an important environmental factor that affects lettuce (Lactuca sativa L.) germination. The present research was conducted to determine the role of seed coverings on lettuce seed germination at high temperature. Five lettuce genotypes were primed in order to bypass thermoinhibitional effects on germination. During germination of primed and nonprimed seeds, imbibition followed a normal triphasic pattern. Primed seeds had higher final water content, a decreased imbibitional phase II, and germinated at 36 °C compared to nonprimed seeds of thermosensitive genotypes, which did not germinate at 36 °C. Puncture tests were conducted to determine the force required to penetrate the whole seed or endosperm of the five genotypes at 24 and 33 °C. `Dark Green Boston', a thermosensitive genotype, had the highest mean resistance (0.207 N) and PI 251245, a thermotolerant genotype, had the lowest (0.139 N). Resistance to penetration of the endopserm of the five genotypes was different at both temperatures. However, three thermotolerant genotypes had lower endosperm resistance than two thermosensitive types. At 36 °C, the penetration force for primed and nonprimed seeds was compared after the first hour of imbibition and 1 hour before radicle protrusion. The force required to penetrate the seed was affected by genotype, seed priming, and duration of imbibition. Puncture force decreased as imbibition time at 36 °C increased in primed and nonprimed seed of each thermotolerant genotype but not in the thermosensitive genotypes. Priming reduced the initial force necessary to penetrate the seed and endosperm in all genotypes. Thus, for radicle protrusion to occur, there must first be a decrease in the resistance of the endosperm layer as evidenced by priming or thermotolerant genotype. Then, the pericarp and integument are sufficiently weakened so that tissue resistance is lower than the turgor pressure of the expanding embryo, allowing germination to be completed.

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Abstract

Dry seeds of common bean, Phaseolus vulgaris L., were treated with 10 and 20 kilo roentgen (kR) of gamma rays to induce plant mutations suitable for use as genetic markers in mapping studies. The 10 and 20 kR treatments produced a total of 9 marker mutations from a total of 412 separate M2 progenies. The mutations changed leaf shape and texture, and produced dwarfism and various chlorophyll deficiencies. Inheritance characteristics were determined and the mutant markers are described. The round leaf (rnd), dark green savoy leaf (dgs), diamond leaf (dia), chlorotic cup leaf (cc), and stipelless lanceolate leaf (sl) mutants are adequately described by their names. Dwarf out-crossing (do) has small leaves, short internodes and pods, and a natural out-crossing frequency of 10%–56%. Chlorotic stem (cs) has a milky white stems, while silver leaf (sil) has its leaf color modified by a silvery reflectance. Progressive chlorosis (pc) has leaves which emerge normal green in color, but become chlorotic with age. The relationship of these mutants to previously reported mutants is discussed.

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Abstract

Nine recessive gamma ray induced bean, Phaseolus vulgaris L., mutants were selected for linkage testing in diallel crosses. All mutants had a common genetic background, Florida dry bean breeding line 7-1404. Linkage was calculated using F2 data, employing Fisher and Balmukand's product ratio method for crosses in repulsion and coupling phases. Repulsion phase linkage tests revealed 2 linkage groups involving 5 genes. Round leaf (rnd), stipelless lanceolate leaf (sl), and dark green savoy leaf (dgs) formed one linkage group, while diamond leaf (dia) and progressive chlorosis (pc) formed the 2nd linkage group. Recombination values from combined repulsion and coupling phase data were: rnd with sl, p = 11.51 ± 0.95; sl with dgs, p = 20.50 ± 1.34; and rnd with dgs, p = 30.07 ± 1.38. Dgs was found to be linked to the yellow wax locus, y, thereby tying the dgs - sl - rnd linkage group into linkage group VII defined by Lamprecht. Also, the y locus was found to be independent of rnd. Preliminary data suggest that the dwarf seed (ds) character may be controlled by the same locus as Lamprecht's tenuis (te), also of linkage group VII, and ds was found to be linked to rnd. If te and ds are identical, then the orientation of the dgs - sl - rnd linkage group with respect to y and te is determined.

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An-assay was developed to determine the level of resistance to the herbicide glyphosate in trangenic seedlings of lettuce. Results of the seedling assay were correlated to results of a similar assay using callus lines of the identical transgenic plants. Transgenic plants were found to be a 32-fold increase in tolerance to glyphosate when compared to wild type plants. This was similar to the response of these transgenic lines in the callus line assay.

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Thermotolerance in lettuce seed at high temperature was investigated using primed and nonprimed seed or seeds matured at 20/10°C and 30/20°C. During seed germination at 36°C, the structural changes of the seed coverings in front of the radicle tip were observed in an anatomical study. In all seeds during imbibition, regardless of seed maturation temperature or priming, a crack appeared on one side of the cap tissue and the endosperm separated from the integument in front of the radicle tip. Additional changes took place during imbibition: the protein bodies in the vacuoles enlarged and were gradually depleted, large empty vacuoles formed, the cytoplasm condensed, the endosperm shrank, the endosperm cell wall dissolved and ruptured, then the radicle elongated toward this ruptured area. The findings suggested that the papery endosperm layer presented mechanical resistance to lettuce seed germination and the weakening of this layer was a prerequisite to radicle protrusion at high temperature. Seeds of `Dark Green Boston', `Everglades', and PI 251245 matured at 30/20°C had greater thermotolerance than those matured at 20/10°C. Results of the anatomical study indicated that the endosperm cell walls in front of the radicle of seeds matured at 30/20°C were more easily disrupted and ruptured during early imbibition than seeds matured at 20/10°C, suggesting that these seeds could germinate quickly at supra-optimal temperatures. From anatomical studies conducted to identify and characterize thermotolerance in lettuce seed germination, it was observed that genotype thermotolerance had the ability to reduce physical resistance of the endosperm by weakening the cell wall and by depleting stored reserves.

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The objectives of these studies were to evaluate the effects of silicon on drought and shade tolerance of st. augustinegrass (Stenotaphrum secundatum). Studies were conducted during 2001 in a glasshouse at the University of Florida Turfgrass Research Envirotron in Gainesville. For both drought and shade evaluations, calcium silicate slag (CaSiO3) was pre-incorporated into pots with commercial potting soil at the rate of 3.36 kg·ha-1 (0.069 lb/1000 ft2). `FX-10' and `FHSA-115' st. augustinegrass were planted into 15.2-cm-diameter × 30.5-cm-deep (6 × 12 inches) plastic pots for the drought study and subjected to minimal irrigation. Under severe drought stress, silicon-amended plants had better responses than non-amended plants. Little improvement was seen under moderate drought stress. `Floratam' and genotype 1997-6 were placed under full sunlight or 50% to 70% shade. There was no benefit from use of silicon under shaded conditions. These findings suggest that silicon might provide improved tolerance to st. augustinegrass under severe drought stress.

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