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Abstract
Using ovaries from dormant flower buds of Rhododendron prinophyllum (Small) Millais as a source for micropropagation, shoot and root regeneration were studied in vitro. Ovaries placed on Anderson's rhododendron shoot tip multiplication medium with 4 mg·liter−1 IAA and 15 mg·liter−1 2iP in darkness for 1 month, then under 16-hr cool-white fluorescent light (35–50 μmol·s−1·m−2) regenerated 20-50 shoots/ovary from ovary wall and calli. After three transfers on Economou and Read's shoot propagation medium, hundreds of shoots were produced per ovary. Shoots 1 to 2 cm in length transferred to root induction media resulted in 90% rooting in 1 to 2 months. Rooted plantlets transplanted to potting mixture had nearly 100% survival. Chemical names used: 1H-indole-3-acetic acid (IAA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP).