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The moisture sorption characteristics of French marigold `Boy Golden' (Tagetes patula), upland aster (Aster ptarmicoides), Texas bluebonnet (Lupinus texenensis), impatiens `Infinity' (Impatiens wallerana), and scarlet sage `Scarlet Pimpernel' (Salvia splendens) was determined by allowing the seeds to equilibrate at different temperatures and relative humidities. The moisture sorption isotherm of the five ornamental species was determined at four different temperatures; i.e., 5, 15, 25, and 35 °C using seven different salt solutions (sodium hydroxide, lithium chloride, magnesium chloride, potassium carbonate, sodium bromide, sodium chloride, and potassium nitrate) having relative humidity ranging from 7% to 96%. The moisture content of the seeds after equilibrium was measured using the gravimetric method. Equilibrium moisture content at 5 °C ranged between 6.2% to 31.5%, 6.2% to 32%, 5.7% to 29.7%, and 6.8% to 16.1% for marigold, upland aster, scarlet sage, and Texas bluebonnet, respectively. At the highest temperature; i.e., 35 °C, the equilibrium moisture content observed ranged between 6.1% to 18.1%, 6% to 21.1%, 6.2% to 20.3%, and 9.1% to 15.6% for marigold, upland aster, scarlet sage and Texas bluebonnet, respectively. The rate of desorption was also determined in the imbibed seeds by drying the seeds in dry cabinet, with water loss recorded by a portable computer inside the cabinet. Total lipid content was also measured to correlate with the water content of the seed.
Germinability and desiccation tolerance are important attributes that seeds acquire during their development. The timing in the expression of these characteristics is important to understand how environmental conditions affecting the mother plant influence seed quality. Lettuce plants (cv. Tango) were cultivated in the greenhouse. Seed germination, under light and darkness, was evaluated in fresh and dry seeds at 3, 5, 7, 9, 11, 13, 15, and 17 days after flowering (DAF). Desiccation was performed ≈1 h after harvest by placing the seeds at 25 °C and ≈53% RH. The seed moisture level after desiccation decreased from ∼14% for 3 DAF seed to ∼7% for 7 DAF seed, and then remained constant until the last sampling. Seeds achieved maximum dry weight (physiological maturity) at ∼13 DAF. Germination of fresh seeds increased from 0% at 3DAF to ∼80% at 5 DAF, reaching 100% at 7 DAF. Dry seeds did not germinate when they were 3 or 5 DAF. Seeds at 7 DAF had ∼10% germination and at 9 DAF ∼100%. When germinated in the dark, an increase in germination from 0% in fresh seeds at 3 DAF to 50% germination at 5 DAF was observed. However, seeds at 9 DAF had dark germination values that decreased to 0% and increased again to ∼70% germination at 13 DAF. Dry seeds had no dark germination until 7 DAF, with variable and low germination (below 20%) until 11 DAF, then germination reached a maximum of ∼55% at 13 DAF and decreased to below 10% at 17 DAF. According to these results, lettuce seed germinability and desiccation tolerance were reached sooner than physiological maturity. In the dark, germination of fresh seeds presented a curve with two peaks suggesting that, depending on the seed developmental stage, two different physiological mechanisms restrict dark germination.