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- Author or Editor: Roberto Rodriguez x
Abstract
Addition of 4–40 μm 2,4-dichlorophenoxyacetic acid (2,4-D) plus 2–6 μm kinetin induced callus formation in fresh cotyledons of walnut (Juglans regia L.), but the presence of phenolic compounds prevented a stable culture. Roots were obtained from callus on a half-strength K(h) medium supplemented with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA) at 40 μm plus kinetin or benzylamino purine (BA) at 2 μm. The origin of roots was usually endogenous.
Abstract
In vitro initiation and development of shoot-buds of walnuts (Juglans regia L.) was obtained on a defined medium using seedlings as a primary explants. Benzylamino purine (BA) at 40 μM induced multiple shoot formation derived from an abnormal conical shoot. Periodic subculture on a fresh media with 0.4 μM of both indolebutyric acid (IBA) and BA resulted in shoots multiplication.
Abstract
Meristem tips of Castanea sativa Mill. from in vitro cultures derived from seedling explants initiated multiple shoot-buds and developed shoot-bud in the presence of 1 mg/liter 6-benzyIamino purine (BA) + 0.01 mg/liter indolebutyric acid (IBA). Root formation was readily achieved within 20 to 25 days when excised single shoots were transferred onto a fresh medium supplemented with IBA (1 mg/liter).
A simple and reliable protocol for plant regeneration from petioles of micropropagated plants of kiwifruit [Actinidia deliciosa (A. Chev) Liang and Ferguson, var. deliciosa `Hayward'] is described. Morphogenic callus was initiated by culturing petioles taken from in vitro-propagated plants. From the media tested, Cheng's K(h) medium plus 0.1 μm IAA, 4.5 μm zeatin, and 2% sucrose was the best for callus induction, maintenance, and shoot bud formation and development. Bases of developed shoots were immersed in 5 mm IBA for 15 seconds; subsequent culture in half-strength K(h) basal medium achieved 82% rooting. Regenerated plantlets were successfully transplanted to soil with 97% survival. Chemical names used: indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); 2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin).