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  • Author or Editor: Roberto Rodríguez x
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Abstract

Meristem tips of Castanea sativa Mill. from in vitro cultures derived from seedling explants initiated multiple shoot-buds and developed shoot-bud in the presence of 1 mg/liter 6-benzyIamino purine (BA) + 0.01 mg/liter indolebutyric acid (IBA). Root formation was readily achieved within 20 to 25 days when excised single shoots were transferred onto a fresh medium supplemented with IBA (1 mg/liter).

Open Access

Abstract

In vitro initiation and development of shoot-buds of walnuts (Juglans regia L.) was obtained on a defined medium using seedlings as a primary explants. Benzylamino purine (BA) at 40 μM induced multiple shoot formation derived from an abnormal conical shoot. Periodic subculture on a fresh media with 0.4 μM of both indolebutyric acid (IBA) and BA resulted in shoots multiplication.

Open Access

Abstract

Addition of 4–40 μm 2,4-dichlorophenoxyacetic acid (2,4-D) plus 2–6 μm kinetin induced callus formation in fresh cotyledons of walnut (Juglans regia L.), but the presence of phenolic compounds prevented a stable culture. Roots were obtained from callus on a half-strength K(h) medium supplemented with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA) at 40 μm plus kinetin or benzylamino purine (BA) at 2 μm. The origin of roots was usually endogenous.

Open Access

A simple and reliable protocol for plant regeneration from petioles of micropropagated plants of kiwifruit [Actinidia deliciosa (A. Chev) Liang and Ferguson, var. deliciosa `Hayward'] is described. Morphogenic callus was initiated by culturing petioles taken from in vitro-propagated plants. From the media tested, Cheng's K(h) medium plus 0.1 μm IAA, 4.5 μm zeatin, and 2% sucrose was the best for callus induction, maintenance, and shoot bud formation and development. Bases of developed shoots were immersed in 5 mm IBA for 15 seconds; subsequent culture in half-strength K(h) basal medium achieved 82% rooting. Regenerated plantlets were successfully transplanted to soil with 97% survival. Chemical names used: indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); 2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin).

Free access